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Formation of eosinophilic and monocytic intradermal inflammatory sites in the dog by injection of human rantes but not human monocyte chemoattractant protein-1, human macrophage inflammatory protein 1-alpha, or human interleukin-8

Academic Article
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Overview

authors

  • Meurer, R.
  • Vanriper, G.
  • Feeney, W.
  • Cunningham, P.
  • Hora, D.
  • Springer, M. S.
  • Macintyre, D. E.
  • Rosen, Hugh

publication date

  • December 1993

journal

  • Journal of Experimental Medicine  Journal

abstract

  • Equilibrium binding studies on canine mononuclear and granulocytic cells allow the identification of a single high affinity receptor for the human C-C chemokine RANTES (dissociation constant, 14 +/- 8 pM), that, in contrast to the human RANTES receptor, has no affinity for human macrophage inflammatory protein 1 alpha (hMIP-1 alpha). A single intradermal injection of hRANTES in dog resulted in eosinophil- and macrophage-rich inflammatory sites within 4 h. Cell infiltration peaked at 16-24 h after hRANTES injection. There was histological evidence of intravascular activation of eosinophils at 4 h, although eosinophils in the vasculature and interstitium contained apparently intact granules. Monocytes were the predominant cells adherent to venular endothelium at 16-24 h. Human MIP-1 alpha elicited no response in canine dermis, whereas monocyte chemoattractant protein 1 caused mild perivascular cuffing with monocytes. In contrast, human interleukin 8 induced a neutrophilic dermal infiltrate that was maximal by 4 h after challenge. This provides the first direct evidence in vivo that RANTES has significant proinflammatory activity and, in addition, could be a mediator in atopic pathologies characterized by eosinophilic and monocytic inflammatory responses.

subject areas

  • Animals
  • Antimicrobial Cationic Peptides
  • Chemokine CCL3
  • Chemokine CCL4
  • Chemokine CCL5
  • Chemotaxis, Leukocyte
  • Cytokines
  • Dogs
  • Dose-Response Relationship, Drug
  • Eosinophils
  • Inflammation
  • Interleukin-8
  • Lymphokines
  • Macrophage Inflammatory Proteins
  • Monocytes
  • Monokines
  • Proteins
  • Receptors, CCR5
  • Receptors, Chemokine
  • Receptors, Immunologic
  • Skin
  • Species Specificity
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Identity

International Standard Serial Number (ISSN)

  • 0022-1007

Digital Object Identifier (DOI)

  • 10.1084/jem.178.6.1913

PubMed ID

  • 7504053
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Additional Document Info

start page

  • 1913

end page

  • 1921

volume

  • 178

issue

  • 6

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