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Specificity of in vitro cyto-toxic t-cell clones directed against vesicular stomatitis-virus

Academic Article
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Overview

authors

  • Rosenthal, K. L.
  • Oldstone, Michael
  • Hengartner, H.
  • Zinkernagel, R. M.

publication date

  • 1983

journal

  • Journal of Immunology  Journal

abstract

  • Cytotoxic T lymphocytes (CTL) generated in mice against a particular serotype of vesicular stomatitis virus (VSV) were previously shown to cross-reactively lyse syngeneic target cells infected with serologically distinct types of VSV. To analyze the antigenic basis of this T cell cross-reactivity, we generated CTL against VSV-Indiana (VSV-Ind) and established them by limiting dilution as cloned in vitro cell lines. The cells continuously proliferate in medium containing concanavalin A-induced T cell growth factors. All of the cells are Thy-1.2+ and Lyt-2.2+. Lysis by these cells is H-2Dd-restricted, no natural killer cell activity is detectable, and all the clones cross-reactively lyse target cells infected with either VSV-Ind or VSV-New Jersey (VSV-NJ). In addition, no specific blocking of primary, secondary, or cloned anti-VSV CTL was achieved with the use of several monoclonal antibodies specific for the glycoprotein of VSV and capable of neutralizing either VSV-Ind or VSV-NJ. These results suggest that VSV serotype-specific neutralizing antibodies may recognize immunodominant determinants of VSV glycoprotein that are distinct from those recognized by the majority of VSV-specific CTL.

subject areas

  • Animals
  • Binding, Competitive
  • Cells, Cultured
  • Clone Cells
  • Cytotoxicity, Immunologic
  • Epitopes
  • Hybridomas
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Serotyping
  • Stomatitis
  • T-Lymphocytes, Cytotoxic
  • Time Factors
  • Vesicular stomatitis Indiana virus
  • Virus Diseases
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Identity

International Standard Serial Number (ISSN)

  • 0022-1767

PubMed ID

  • 6190924
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Additional Document Info

start page

  • 475

end page

  • 478

volume

  • 131

issue

  • 1

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