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Metalloantibodies: Mercury(II)-dependent acyl transferases

Academic Article
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Overview

authors

  • Brummer, O.
  • Hoffman, T. Z.
  • Janda, Kim

publication date

  • September 2001

journal

  • Bioorganic & Medicinal Chemistry  Journal

abstract

  • A new approach for the elicitation of metal-dependent catalytic antibodies for ester hydrolysis is described. A coordinatively unsaturated mercury complex 1-(Hg), has been utilized as a hapten to elicit antibodies that incorporate mercury(II) as a Lewis acid cofactor. From a panel of monoclonal antibodies generated to 1-(Hg), antibody 38G2 was found to hydrolyze the ester 3 in the presence of HgCl(2) [K(m)app(3)=345 microM; K(m)app(Hg(2+))=87 microM; k(cat)app/k(uncat)=3 x 10(2)]. This is the first example of a biocatalyst that enlists mercuric ion as a cofactor and it is anticipated that this approach will open new avenues for exploitation of metals thought previously beyond the scope of protein catalysts.

subject areas

  • Acyltransferases
  • Antibodies, Catalytic
  • Antibodies, Monoclonal
  • Enzyme Inhibitors
  • Haptens
  • Hydrolysis
  • Inhibitory Concentration 50
  • Kinetics
  • Mercury
  • Metals
  • Models, Molecular
  • Phosphates
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Identity

International Standard Serial Number (ISSN)

  • 0968-0896

Digital Object Identifier (DOI)

  • 10.1016/s0968-0896(01)00089-x

PubMed ID

  • 11553463
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Additional Document Info

start page

  • 2253

end page

  • 2257

volume

  • 9

issue

  • 9

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