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Formation of prostamides from anandamide in FAAH knockout mice analyzed by HPLC with tandem mass spectrometry

Academic Article
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Overview

authors

  • Weber, A.
  • Ni, J. S.
  • Ling, K. H. J.
  • Acheampong, A.
  • Tang-Liu, D. D. S.
  • Burk, R.
  • Cravatt, Benjamin
  • Woodward, D.

publication date

  • April 2004

journal

  • Journal of Lipid Research  Journal

abstract

  • We investigated the formation of PGF(2alpha) 1-ethanolamide, PGE(2) 1-ethanolamide, and PGD(2) 1-ethanolamide (prostamides F(2alpha), E(2), and D(2), respectively) in liver, lung, kidney, and small intestine after a single intravenous bolus administration of 50 mg/kg of anandamide to normal and fatty acid amide hydrolase knockout (FAAH -/-) male mice. One group of three normal mice was not dosed (naïve) while another group of three normal mice received a bolus intravenous injection of 50 mg/kg of anandamide. Three FAAH -/- mice also received an intravenous injection of 50 mg/kg of anandamide. After 30 min, the lung, liver, kidney, and small intestine were harvested and processed by liquid-liquid extraction. The concentrations of prostamide F(2alpha), prostamide E(2), prostamide D(2), and anandamide were determined by HPLC-tandem mass spectrometry. Prostamide F(2alpha) was detected in tissues in FAAH -/- mice after administration of anandamide. Concentrations of anandamide, prostamide E(2), and prostamide D(2) in liver, kidney, lung, and small intestine were much higher in the anandamide-treated FAAH -/- mice than those of the anandamide-treated control mice. This report demonstrates that prostamides, including prostamide F(2alpha), were formed in vivo from anandamide, potentially by the cyclooxygenase-2 pathway when the competing FAAH pathway is lacking.

subject areas

  • Amidohydrolases
  • Animals
  • Arachidonic Acids
  • Biotransformation
  • Chromatography, High Pressure Liquid
  • Cyclooxygenase 2
  • Dinoprostone
  • Endocannabinoids
  • Intestine, Small
  • Isoenzymes
  • Kidney
  • Liver
  • Lung
  • Mass Spectrometry
  • Mice
  • Mice, Knockout
  • Polyunsaturated Alkamides
  • Prostaglandin-Endoperoxide Synthases
  • Prostaglandins
  • Tissue Distribution
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Research

keywords

  • PGD(2) 1-ethanolamide
  • PGE(2) 1-ethanolamide
  • PGF(2 alpha) 1-ethanolamide
  • anandamide
  • cyclooxygenase-2
  • fatty acid amide hydrolase
  • high-performance liquid chromatography
  • prostamide D-2
  • prostamide E-2
  • prostamide F-2 alpha
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Identity

International Standard Serial Number (ISSN)

  • 0022-2275

Digital Object Identifier (DOI)

  • 10.1194/jlr.M300475-JLR200

PubMed ID

  • 14729864
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Additional Document Info

start page

  • 757

end page

  • 763

volume

  • 45

issue

  • 4

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