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Chemo-resistant protein expression pattern of glioblastoma cells (A172) to perillyl alcohol

Academic Article
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Overview

authors

  • Fischer, J. D. D.
  • Carvalho, P. C.
  • da Fonseca, C. O.
  • Liao, L. J.
  • Degrave, W. M.
  • Carvalho, M. D. D.
  • Yates III, John
  • Domont, G. B.

publication date

  • January 2011

journal

  • Journal of Proteome Research  Journal

abstract

  • Glioblastoma multiform (GBM) is by far the most malignant glioma. We have introduced a new treatment for GBMs that comprises the inhalation of a naturally occurring terpene with chemotherapeutic properties known as perillyl alcohol (POH). Clinical trial results on recurrent GBM patients showed that POH extends the average life by more than eight months, temporarily slows tumor growth, and in some cases even decreases tumor size. After approximately seven months, the tumor continues to grow and leads to a dismal prognosis. To investigate how these tumors become resistant to POH, we generated an A172 human glioblastoma cell culture tolerant to 0.06 mM of POH (A172r). We used Multidimensional Protein Identification Technology (MudPIT) to compare the protein expression profile of A172r cells to the established glioblastoma A172 cell line. Our results include a list of identified proteins unique to either the resistant or the nonresistant cell line. These proteins are related to cellular growth, negative apoptosis regulation, Ras pathway, and other key cellular functions that could be connected to the underlying mechanisms of resistance.

subject areas

  • Antineoplastic Agents
  • Blotting, Western
  • Brain
  • Cell Line, Tumor
  • Clinical Trials as Topic
  • Drug Resistance, Neoplasm
  • Electrophoresis, Gel, Two-Dimensional
  • Glioblastoma
  • Humans
  • Magnetic Resonance Imaging
  • Monoterpenes
  • Peptide Fragments
  • Proteome
  • Proteomics
  • Reproducibility of Results
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Research

keywords

  • A172 cells
  • MudPIT
  • glioblastoma
  • perillyl alcohol
  • protein profile
  • resistant
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Identity

PubMed Central ID

  • PMC3017632

International Standard Serial Number (ISSN)

  • 1535-3893

Digital Object Identifier (DOI)

  • 10.1021/pr100677g

PubMed ID

  • 20806975
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Additional Document Info

start page

  • 153

end page

  • 160

volume

  • 10

issue

  • 1

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