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Identification and characterization of genes upregulated in cells transformed by v-jun

Academic Article
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Overview

authors

  • Fu, S. L.
  • Waha, A.
  • Vogt, Peter K.

publication date

  • July 2000

journal

  • Oncogene  Journal

abstract

  • The transcription factor Jun (c-Jun) functions as a recipient of extracellular growth signals and converts them into patterns of gene expression. An oncogenic variant of c-Jun was isolated from the acutely transforming retrovirus ASV17. Overexpression of this viral Jun (v-Jun) induces transformation of chicken embryo fibroblasts (CEF) in culture and fibrosarcomas in chickens. v-Jun is a constitutively active form of c-Jun and transforms cells presumably by deregulating the expression of specific target genes. In this report, we describe six genes whose transcripts are upregulated in v-Jun-transformed CEF. Three of these genes show homology to known mammalian genes, to MAP kinase phosphatase 2 (MKP-2), to reversion-induced LIM protein (RIL) and to cytokine-inducible SH2-containing protein (CIS). Northern blot analysis, using CEF infected with various Jun mutants or an estrogen-regulatable Jun chimera, revealed distinct induction patterns of individual targets by v-Jun. The chicken RIL homolog showed an expression pattern tightly correlated with the activity of v-Jun. Its expression is also transformation-dependent, suggesting a role for this gene in v-Jun transformation. The newly identified v-Jun targets can serve as molecular markers in the v-Jun transformation process. Oncogene (2000) 19, 3537 - 3545

subject areas

  • Amino Acid Sequence
  • Animals
  • Blotting, Northern
  • Cell Line, Transformed
  • Cell Transformation, Viral
  • Chick Embryo
  • DNA, Complementary
  • DNA-Binding Proteins
  • Dual-Specificity Phosphatases
  • Fibroblasts
  • Gene Expression Regulation, Neoplastic
  • Gene Expression Regulation, Viral
  • Gene Library
  • Genes, jun
  • Genetic Vectors
  • Humans
  • Immediate-Early Proteins
  • LIM Domain Proteins
  • Mice
  • Microfilament Proteins
  • Mitogen-Activated Protein Kinase Phosphatases
  • Molecular Sequence Data
  • Neoplasm Proteins
  • Oncogene Protein p65(gag-jun)
  • Polymerase Chain Reaction
  • Protein Phosphatase 2
  • Protein Tyrosine Phosphatases
  • Recombinant Fusion Proteins
  • Retroviridae
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Species Specificity
  • Subtraction Technique
  • Suppressor of Cytokine Signaling Proteins
  • Transcription, Genetic
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Research

keywords

  • oncogenic transformation
  • transcriptional activation
  • transcriptional targets
  • v-Jun
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Identity

International Standard Serial Number (ISSN)

  • 0950-9232

Digital Object Identifier (DOI)

  • 10.1038/sj.onc.1203691

PubMed ID

  • 10918612
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Additional Document Info

start page

  • 3537

end page

  • 3545

volume

  • 19

issue

  • 31

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