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Detection of calcium transients in Drosophila mushroom body neurons with camgaroo reporters

Academic Article
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Overview

authors

  • Yu, D.
  • Baird, G. S.
  • Tsien, R. Y.
  • Davis, Ronald

publication date

  • January 2003

journal

  • Journal of Neuroscience  Journal

abstract

  • Camgaroos are yellow fluorescent protein derivatives that hold promise as transgenically encoded calcium sensors in behaving animals. We expressed two versions of camgaroo in Drosophila mushroom bodies using the galactosidase-4 (GAL4) system. Potassium depolarization of brains expressing the reporters produces a robust increase in fluorescence that is blocked by removing extracellular calcium or by antagonists of voltage-dependent calcium channels. The fluorescence increase is not attributable to cytoplasmic alkalization; depolarization induces a slight acidification of the cytoplasm of mushroom body neurons. Acetylcholine applied near the dendrites of the mushroom body neurons induces a rapid and ipsilateral-specific fluorescence increase in the mushroom body axons that is blocked by antagonists of calcium channels or nicotinic acetylcholine receptors. Fluorescence was observed to increase in all three classes of mushroom body neurons, indicating that all types respond to cholinergic innervation.

subject areas

  • Acetylcholine
  • Animals
  • Bacterial Proteins
  • Calcium
  • Calcium Channel Blockers
  • Cells, Cultured
  • DNA-Binding Proteins
  • Drosophila
  • Green Fluorescent Proteins
  • Hydrogen-Ion Concentration
  • Indicators and Reagents
  • Luminescent Proteins
  • Microscopy, Fluorescence
  • Mushroom Bodies
  • Neurons
  • Potassium
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors
  • Transgenes
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Research

keywords

  • Drosophila
  • GFP
  • calcium
  • camgaroos
  • mushroom bodies
  • optical imaging
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Identity

International Standard Serial Number (ISSN)

  • 0270-6474

PubMed ID

  • 12514202
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Additional Document Info

start page

  • 64

end page

  • 72

volume

  • 23

issue

  • 1

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