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An improved ELISA for anti-native DNA by elimination of interference by anti-histone antibodies

Academic Article
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Overview

authors

  • Rubin, R. L.
  • Joslin, F. G.
  • Tan, Eng

publication date

  • 1983

journal

  • Journal of Immunological Methods  Journal

abstract

  • We evaluated an enzyme-linked immunosorbent assay (ELISA) for antibodies to native DNA (nDNA) in which protamine was used to link DNA to polystyrene. Elevated anti-nDNA was largely restricted to patients with systemic lupus erythematosus (SLE), and within this group good correlation between ELISA and the ammonium sulfate assay was obtained. However, substantial background immunoglobulin binding to protamine coated wells was commonly observed, and it was necessary to subtract this activity from each anti-DNA determination. Many of the SLE sera also contained anti-histone antibodies, and this antibody activity showed significant correlation with the binding to protamine. In contrast, methylated bovine serum albumin (mBSA) did not bind anti-histone antibodies and provided a substrate for coupling nDNA to polystyrene. This modified ELISA allowed the quantitation of antibodies to native DNA without the simultaneous binding of anti-histone antibodies.

subject areas

  • Ammonium Sulfate
  • Antibodies, Antinuclear
  • DNA
  • Enzyme-Linked Immunosorbent Assay
  • Histones
  • Humans
  • Immunoenzyme Techniques
  • Isoelectric Point
  • Lupus Erythematosus, Systemic
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Identity

International Standard Serial Number (ISSN)

  • 0022-1759

Digital Object Identifier (DOI)

  • 10.1016/s0022-1759(83)80009-x

PubMed ID

  • 6355300
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Additional Document Info

start page

  • 359

end page

  • 366

volume

  • 63

issue

  • 3

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