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E2A proteins are required for proper B-cell development and initiation of immunoglobulin gene rearrangements

Academic Article
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Overview

authors

  • Bain, G.
  • Maandag, E. C. R.
  • Izon, D. J.
  • Amsen, D.
  • Kruisbeek, A. M.
  • Weintraub, B. C.
  • Krop, I.
  • Schlissel, M. S.
  • Feeney, Ann
  • Vanroon, M.
  • Vandervalk, M.
  • Teriele, H. P. J.
  • Berns, A.
  • Murre, C.

publication date

  • December 1994

journal

  • Cell  Journal

abstract

  • E12 and E47 are two helix-loop-helix transcription factors that arise by alternative splicing of the E2A gene. Both have been implicated in the regulation of immunoglobulin gene expression. We have now generated E2A (-/-) mice by gene targeting. E2A-null mutant mice fail to generate mature B cells. The arrest of B cell development occurs at an early stage, since no immunoglobulin DJ rearrangements can be detected in homozygous mutant mice. While immunoglobulin germline I mu RAG-1, mb-1, CD19, and lambda 5 transcripts are dramatically reduced in fetal livers of E2A (-/-) mice, B29 and mu degrees transcripts are present, but at lower levels. In addition, we show that Pax-5 transcripts are significantly reduced in fetal livers of E2A (-/-) mice. These data suggest a crucial role for E2A products as central regulators in early B cell differentiation.

subject areas

  • Animals
  • B-Lymphocytes
  • Base Sequence
  • Cell Differentiation
  • DNA-Binding Proteins
  • Gene Rearrangement
  • Genes, Immunoglobulin
  • Homeodomain Proteins
  • Homozygote
  • Immunoglobulin lambda-Chains
  • Lymphoid Tissue
  • Mice
  • Mice, Mutant Strains
  • Molecular Sequence Data
  • Proteins
  • RNA, Messenger
  • TCF Transcription Factors
  • Transcription Factor 7-Like 1 Protein
  • Transcription Factors
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Identity

International Standard Serial Number (ISSN)

  • 0092-8674

Digital Object Identifier (DOI)

  • 10.1016/0092-8674(94)90077-9

PubMed ID

  • 8001125
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Additional Document Info

start page

  • 885

end page

  • 892

volume

  • 79

issue

  • 5

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