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An isothermal system that couples ligand-dependent catalysis to ligand-independent exponential amplification

Academic Article
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Overview

authors

  • Lam, B. J.
  • Joyce, Gerald

publication date

  • March 2011

journal

  • Journal of the American Chemical Society  Journal

abstract

  • A system was devised that enables quantitative, ligand-dependent exponential amplification for various ligands that can be recognized by an RNA aptamer. The aptamer is linked to an RNA enzyme that catalyzes the joining of two oligonucleotide substrates. The product of this reaction is another RNA enzyme that undergoes self-sustained replication at constant temperature, increasing in copy number exponentially. The concentration of the ligand determines the amount of time required for the replication products to reach a threshold concentration. A standardized plot of time to threshold versus ligand concentration can be used to determine the concentration of ligand in an unknown sample. This system is analogous to quantitative polymerase chain reaction (PCR), linking rare recognition events to subsequent exponential amplification, but unlike PCR can be applied to the quantitative detection of non-nucleic acid ligands.

subject areas

  • Aptamers, Nucleotide
  • Bacteriophages
  • Base Sequence
  • Biosensing Techniques
  • Catalysis
  • DNA-Directed DNA Polymerase
  • DNA-Directed RNA Polymerases
  • Ligands
  • Molecular Sequence Data
  • Nucleic Acid Amplification Techniques
  • Sensitivity and Specificity
  • Theophylline
  • Thermus
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Identity

PubMed Central ID

  • PMC3048896

International Standard Serial Number (ISSN)

  • 0002-7863

Digital Object Identifier (DOI)

  • 10.1021/ja111136d

PubMed ID

  • 21322594
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Additional Document Info

start page

  • 3191

end page

  • 3197

volume

  • 133

issue

  • 9

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