Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form

The transition-state transcription regulator abrb of bacillus-subtilis is autoregulated during vegetative growth

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Strauch, M. A.
  • Perego, Marta
  • Burbulys, D.
  • Hoch, James

publication date

  • 1989

journal

  • Molecular Microbiology  Journal

abstract

  • The DNA-binding AbrB protein of Bacillus subtilis is an ambiactive transcriptional regulator of genes expressed during the transition state between vegetative growth and the onset of stationary phase and sporulation. Studies on the transcriptional control of AbrB synthesis using abrB-lacZ fusions indicated that the abrB gene was autoregulated. This was consistent with the observation that purified AbrB protein bound specifically to the promoter region of its own gene in DNase I protection experiments. The structural gene mutation abrB4 abolished the autoregulation and purified AbrB4 protein did not have the promoter binding properties associated with the wild-type protein. Both AbrB and AbrB4 proteins were shown to be hexamers of 10,500 Dalton subunits and subunit exchange occurred between the proteins in vitro. However, the presence of only one or two mutant subunits dramaticaly altered the DNA-binding ability of the multimeric protein. The results support a model in which autoregulation of the abrB gene is an important factor in preventing sporulation-associated genes from being expressed during vegetative growth.

subject areas

  • Bacillus subtilis
  • Bacterial Proteins
  • Chromatography, Gel
  • DNA-Binding Proteins
  • Deoxyribonuclease I
  • Molecular Weight
  • Mutation
  • Plasmids
  • Recombinant Fusion Proteins
  • Transcription Factors
  • Transcription, Genetic
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 0950-382X

Digital Object Identifier (DOI)

  • 10.1111/j.1365-2958.1989.tb00270.x

PubMed ID

  • 2507867
scroll to property group menus

Additional Document Info

start page

  • 1203

end page

  • 1209

volume

  • 3

issue

  • 9

©2019 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support