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Alanine scanning mutagenesis of an alpha beta T cell receptor: mapping the energy of antigen recognition

Academic Article
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Overview

authors

  • Manning, T. C.
  • Schlueter, C. J.
  • Brodnicki, T. C.
  • Parke, E. A.
  • Speir, J. A.
  • Garcia, K. C.
  • Teyton, Luc
  • Wilson, Ian
  • Kranz, D. M.

publication date

  • April 1998

journal

  • Immunity  Journal

abstract

  • The T cell receptor (TCR) from the alloreactive T lymphocyte 2C recognizes a nonamer peptide QL9 complexed with the MHC class I molecule H2-Ld. Forty-two single-site alanine substitutions of the 2C TCR were analyzed for binding to QL9/Ld and anti-TCR antibodies. The results provided a detailed energy map of T cell antigen recognition and indicated that the pMHC and clonotypic antibody epitopes on the TCR were similar. Although residues in each Valpha and Vbeta CDR are important in binding pMHC, the most significant energy for the TCR/QL9/Ld interaction was contributed by CDRs 1 and 2 of both alpha and beta chains. The extent to which the individual energy contributions are directed at class I helices or peptide was also assessed.

subject areas

  • Alanine
  • Amino Acid Sequence
  • Amino Acid Substitution
  • Animals
  • Antibodies
  • Binding Sites
  • Epitopes
  • H-2 Antigens
  • Histocompatibility Antigen H-2D
  • In Vitro Techniques
  • Mice
  • Models, Molecular
  • Mutagenesis, Site-Directed
  • Protein Conformation
  • Protein Folding
  • Receptors, Antigen, T-Cell, alpha-beta
  • T-Lymphocytes
  • Thermodynamics
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Identity

International Standard Serial Number (ISSN)

  • 1074-7613

Digital Object Identifier (DOI)

  • 10.1016/s1074-7613(00)80547-6

PubMed ID

  • 9586632
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Additional Document Info

start page

  • 413

end page

  • 425

volume

  • 8

issue

  • 4

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