Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form

Splice site selection in the proteolipid protein (PLP) gene transcript and primary structure of the DM-20 protein of central nervous system myelin

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Bloom, Floyd
  • Lai, C.
  • Milner, R, J.
  • Nave, K. A.

publication date

  • August 1987

journal

  • Proceedings of the National Academy of Sciences of the United States of America  Journal

abstract

  • Proteolipid protein (PLP) is the major myelin membrane protein of the central nervous system. We have isolated a copy of an alternatively spliced PLP gene transcript from a mouse brain cDNA library that was screened for PLP-related sequences. The encoded 241-amino acid protein differs from PLP by an internal deletion of 35-amino acid residues (116-150) from the major hydrophilic domain. This PLP variant is identical with the DM-20 protein of myelin, previously described as a brain-specific myelin component and known to be related to PLP. We determined the corresponding nucleotide sequence of the rat PLP gene and found that DM-20 mRNA results when a second 5' splice site, located 105 nucleotides within the third exon of the primary PLP transcript, is utilized in precursor mRNA (pre-mRNA) splicing. This demonstrates that alternative 5' splice site selection can determine the protein product of a cellular gene. DM-20 mRNA is expressed in rat brain with approximately 50% abundance relative to PLP mRNA and appears to be developmentally coregulated.

subject areas

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Mice
  • Mice, Jimpy
  • Myelin Proteins
  • Myelin Proteolipid Protein
  • Myelin Sheath
  • Nerve Tissue Proteins
  • Protein Conformation
  • Proteolipids
  • RNA Splicing
  • RNA, Messenger
  • Rats
  • Rats, Inbred Strains
  • Transcription, Genetic
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.84.16.5665

PubMed ID

  • 2441390
scroll to property group menus

Additional Document Info

start page

  • 5665

end page

  • 5669

volume

  • 84

issue

  • 16

©2021 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support