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Human combinatorial antibody libraries to hepatitis-b surface-antigen

Academic Article
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Overview

authors

  • Zebedee, S. L.
  • Barbas III, Carlos
  • Hom, Y. L.
  • Caothien, R. H.
  • Graff, R.
  • Degraw, J.
  • Pyati, J.
  • Lapolla, R.
  • Burton, Dennis
  • Lerner, Richard
  • Thornton, G. B.

publication date

  • April 1992

journal

  • Proceedings of the National Academy of Sciences of the United States of America  Journal

abstract

  • Human antibody Fab fragments that bind to hepatitis B surface antigen (HBsAg) were generated by using a recombinant phage surface-display expression system. Characterization of HBsAg-specific Fab fragments isolated from two vaccinated individuals reveals diversity in specificity of antigen binding and in the sequences of the complementarity-determining region. The sequence results show examples of human light-chain promiscuity that result in fine specificity changes and a strong relationship to a human germ-line gene. This application illustrates further that this technique is a powerful tool to isolate distinct human antibodies against immunogenic viral targets.

subject areas

  • Amino Acid Sequence
  • Base Sequence
  • DNA
  • Enzyme-Linked Immunosorbent Assay
  • Gene Library
  • Hepatitis B Antibodies
  • Hepatitis B Surface Antigens
  • Humans
  • Immunoglobulin Fab Fragments
  • Immunoglobulin Heavy Chains
  • Immunoglobulin Light Chains
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides
  • RNA
  • Recombinant Proteins
  • Sequence Homology, Nucleic Acid
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Identity

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.89.8.3175

PubMed ID

  • 1373487
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Additional Document Info

start page

  • 3175

end page

  • 3179

volume

  • 89

issue

  • 8

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