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A specific assay for anti-hla antibodies - application to platelet donor selection

Academic Article
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Overview

authors

  • Millard, F. E.
  • Tani, P.
  • Mc Millan, Robert

publication date

  • November 1987

journal

  • Blood  Journal

abstract

  • Alloimmunization to donor class I HLA antigens represents a major obstacle to successful platelet transfusion therapy. It is desirable to distinguish alloimmunization from nonimmunologic causes of poor platelet survival to assess the need for HLA-matched, single-donor platelets. We describe a new in vitro assay for anti-HLA antibodies and report its application to the problem of platelet crossmatching. In contrast to previously described crossmatch techniques, the immunobead assay is specific for anti-HLA antibodies. The assay was used to evaluate 51 single-donor platelet transfusions given to seven patients from 35 different donors. Recipient plasma was assayed for antibodies directed against HLA antigens present on donor platelets. A one-hour posttransfusion corrected count increment of greater than or equal to 7,500 was considered a successful outcome. Twenty-nine of 33 (87.9%) transfusion episodes associated with a negative immunobead assay had successful outcomes. The four unsuccessful transfusions were associated with potential nonimmunologic causes of poor platelet survival. Only two of 18 (11.1%) episodes associated with a positive assay had successful outcomes. Only one unsuccessful transfusion episode was associated with a negative immunobead assay and a positive radiolabeled antiglobulin test result, which suggested that isolated alloantibodies to antigens other than class I HLA antigens are not a common cause of platelet refractoriness. Platelets stored in suspension at 4 degrees C or frozen in liquid nitrogen were found suitable for crossmatch testing.

subject areas

  • Adult
  • Aged
  • Autoantibodies
  • Blood Component Removal
  • Blood Donors
  • Blood Platelets
  • Bone Marrow Transplantation
  • Female
  • HLA Antigens
  • Humans
  • Leukemia
  • Leukemia, Lymphoid
  • Leukemia, Myeloid, Acute
  • Male
  • Middle Aged
  • Platelet Transfusion
  • Plateletpheresis
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Identity

International Standard Serial Number (ISSN)

  • 0006-4971

PubMed ID

  • 3311206
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Additional Document Info

start page

  • 1495

end page

  • 1499

volume

  • 70

issue

  • 5

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