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Monoclonal-antibodies specific for beta-adrenergic ligands

Academic Article
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Overview

authors

  • Chamat, S.
  • Hoebeke, J.
  • Strosberg, Donny

publication date

  • 1984

journal

  • Journal of Immunology  Journal

abstract

  • After somatic cell fusion between splenocytes of immunized BALB/c mice and NS-1 myeloma cells, eight clones were obtained secreting anti-alprenolol antibodies as characterized by means of an ELISA. Four of these were subcloned and were studied further. The association constant for alprenolol ranged from 1.9 X 10(6) M-1 to 24 to 10(6) M-1. Competitive inhibition of [3H]-l-dihydroalprenolol binding revealed cross-reactivity with beta-adrenergic ligands, with a higher avidity for antagonists than for agonists. Two of the antibodies had a higher affinity for the l-isomer than for the d-isomer. The most stereospecific of these antibodies showed only affinity for beta-adrenergic antagonists and for the agonist isoproterenol. The other recognized both beta-adrenergic antagonists and agonists; it also showed an increase in tryptophan fluorescence after ligand binding. This property was used for the physicochemical study of the hapten-antibody interaction.

subject areas

  • Alprenolol
  • Animals
  • Antibodies, Monoclonal
  • Antibody Specificity
  • Binding Sites, Antibody
  • Binding, Competitive
  • Dihydroalprenolol
  • Mice
  • Mice, Inbred BALB C
  • Propranolol
  • Spectrometry, Fluorescence
  • Thermodynamics
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Identity

International Standard Serial Number (ISSN)

  • 0022-1767

PubMed ID

  • 6747296
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Additional Document Info

start page

  • 1547

end page

  • 1552

volume

  • 133

issue

  • 3

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