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Mapping of a functional autoimmune epitope on the beta-1-adrenergic receptor in patients with idiopathic dilated cardiomyopathy

Academic Article
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Overview

authors

  • Magnusson, Y.
  • Marullo, S.
  • Hoyer, S.
  • Waagstein, F.
  • Andersson, B.
  • Vahlne, A.
  • Guillet, J. G.
  • Strosberg, Donny
  • Hjalmarson, A.
  • Hoebeke, J.

publication date

  • November 1990

journal

  • Journal of Clinical Investigation  Journal

abstract

  • The presence and properties of serum autoantibodies against beta-adrenergic receptors in patients with idiopathic dilated cardiomyopathy were studied using synthetic peptides derived from the predicted sequences of the human beta-adrenergic receptors. Peptides corresponding to the sequences of the second extracellular loop of the human beta 1- and beta 2-adrenergic receptors were used as antigens in an enzyme immunoassay to screen sera from patients with dilated cardiomyopathy (n = 42), ischemic heart disease (n = 17), or healthy blood donors (n = 34). The sera of thirteen dilated cardiomyopathy patients, none of the ischemic heart disease patients, and four of the healthy controls monospecifically recognized the beta 1-peptide. Only affinity-purified antibodies of these patients had a inhibitory effect on radioligand binding to the beta 1 receptor of C6 rat glioma cells. They recognized the receptor protein by immunoblot and bound in situ to human myocardial tissue. We conclude that a subgroup of patients with idiopathic dilated cardiomyopathy have in their sera autoantibodies specifically directed against the second extracellular loop of the beta 1-adrenergic receptor. These antibodies could serve as a marker of an autoimmune response with physiological and/or pathological implications.

subject areas

  • Adult
  • Aged
  • Amino Acid Sequence
  • Antibody Affinity
  • Autoantibodies
  • Autoimmune Diseases
  • Cardiomyopathy, Dilated
  • Epitopes
  • Female
  • Humans
  • Immunoenzyme Techniques
  • Male
  • Middle Aged
  • Molecular Sequence Data
  • Receptors, Adrenergic, beta
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Identity

International Standard Serial Number (ISSN)

  • 0021-9738

Digital Object Identifier (DOI)

  • 10.1172/jci114888

PubMed ID

  • 1700798
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Additional Document Info

start page

  • 1658

end page

  • 1663

volume

  • 86

issue

  • 5

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