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Selection and characterization of a human monoclonal neutralizing antibody for clostridium Botulinum Neurotoxin serotype B

Academic Article
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Overview

authors

  • Janda, Kim
  • Zhou, H. Y.
  • Johnson, E. A.
  • Pellett, S.
  • Zhou, Bin

publication date

  • February 2009

journal

  • Bioorganic & Medicinal Chemistry Letters  Journal

abstract

  • Botulinum neurotoxins (BoNTs) are causative agents for botulism and are identified as a category A bioterror agents by the Centers for Disease Control and Prevention (CDC). Current antitoxins against BoNTs intoxication have some limitations including side effects or limited supply. As an alternative, neutralizing monoclonal antibodies will play an increasing role as BoNTs therapeutics. To date, no human anti-BoNT/B neutralizing monoclonal antibodies have yet to be reported. Herein, we describe an improved selection approach and characterization of a human monoclonal antibody, F2, which is capable of binding BoNT/B with high specificity and displays neutralizing activity in an in vitro cell-based assay. Through surface plasmon resonance studies, we have determined its association and dissociation rate constants. In sum, our data demonstrate that monoclonal antibody F2 is a promising BoNT/B therapeutic lead for further development.

subject areas

  • Animals
  • Antibodies, Monoclonal
  • Antitoxins
  • Botulinum Toxins
  • Botulinum Toxins, Type A
  • Botulism
  • Cells, Cultured
  • Clostridium botulinum
  • Humans
  • Kinetics
  • Neutralization Tests
  • Peptide Library
  • Rats
  • Spinal Cord
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Research

keywords

  • Botulinum neurotoxin
  • Monoclonal antibody
  • Neutralizing antibody
  • Phage display
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Identity

PubMed Central ID

  • PMC2645068

International Standard Serial Number (ISSN)

  • 0960-894X

Digital Object Identifier (DOI)

  • 10.1016/j.bmcl.2008.12.055

PubMed ID

  • 19112017
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Additional Document Info

start page

  • 662

end page

  • 664

volume

  • 19

issue

  • 3

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