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Protein microarrays: Prospects and problems

Academic Article
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Overview

authors

  • Kodadek, Thomas

publication date

  • February 2001

journal

  • Chemistry & Biology  Journal

abstract

  • Protein microarrays are potentially powerful tools in biochemistry and molecular biology. Two types of protein microarrays are defined. One, termed a protein function array, will consist of thousands of native proteins immobilized in a defined pattern. Such arrays can be utilized for massively parallel testing of protein function, hence the name. The other type is termed a protein-detecting array. This will consist of large numbers of arrayed protein-binding agents. These arrays will allow for expression profiling to be done at the protein level. In this article, some of the major technological challenges to the development of protein arrays are discussed, along with potential solutions.

subject areas

  • Ligands
  • Protein Binding
  • Proteins
  • Surface Plasmon Resonance
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Research

keywords

  • microarray
  • protein array
  • protein immobilization
  • protein-protein interaction
  • surface plasmon resonance
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Identity

International Standard Serial Number (ISSN)

  • 1074-5521

Digital Object Identifier (DOI)

  • 10.1016/s1074-5521(00)90067-x

PubMed ID

  • 11251285
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Additional Document Info

start page

  • 105

end page

  • 115

volume

  • 8

issue

  • 2

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