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TRPV3 in keratinocytes transmits temperature information to sensory neurons via ATP

Academic Article
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Overview

authors

  • Mandadi, S.
  • Sokabe, T.
  • Shibasaki, K.
  • Katanosaka, K.
  • Mizuno, A.
  • Moqrich, A.
  • Patapoutian, Ardem
  • Fukumi-Tominaga, T.
  • Mizumura, K.
  • Tominaga, M.

publication date

  • October 2009

journal

  • Pflugers Archiv-European Journal of Physiology  Journal

abstract

  • Transient receptor potential V3 (TRPV3) and TRPV4 are heat-activated cation channels expressed in keratinocytes. It has been proposed that heat-activation of TRPV3 and/or TRPV4 in the skin may release diffusible molecules which would then activate termini of neighboring dorsal root ganglion (DRG) neurons. Here we show that adenosine triphosphate (ATP) is such a candidate molecule released from keratinocytes upon heating in the co-culture systems. Using TRPV1-deficient DRG neurons, we found that increase in cytosolic Ca(2+)-concentration in DRG neurons upon heating was observed only when neurons were co-cultured with keratinocytes, and this increase was blocked by P2 purinoreceptor antagonists, PPADS and suramin. In a co-culture of keratinocytes with HEK293 cells (transfected with P2X(2) cDNA to serve as a bio-sensor), we observed that heat-activated keratinocytes secretes ATP, and that ATP release is compromised in keratinocytes from TRPV3-deficient mice. This study provides evidence that ATP is a messenger molecule for mainly TRPV3-mediated thermotransduction in skin.

subject areas

  • Adenosine Triphosphate
  • Animals
  • Calcium
  • Cells, Cultured
  • Coculture Techniques
  • Ganglia, Spinal
  • Glutamic Acid
  • Hot Temperature
  • Humans
  • Keratinocytes
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Patch-Clamp Techniques
  • Sensory Receptor Cells
  • Serotonin
  • Signal Transduction
  • Skin
  • TRPV Cation Channels
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Research

keywords

  • ATP
  • Keratinocytes
  • TRP channel
  • TRPV3
  • TRPV4
  • Thermosensation
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Identity

PubMed Central ID

  • PMC2745623

International Standard Serial Number (ISSN)

  • 0031-6768

Digital Object Identifier (DOI)

  • 10.1007/s00424-009-0703-x

PubMed ID

  • 19669158
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Additional Document Info

start page

  • 1093

end page

  • 1102

volume

  • 458

issue

  • 6

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