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Activity-based protein profiling reagents for protein arginine deiminase 4 (pad4): Synthesis and in vitro evaluation of a fluorescently labeled probe

Academic Article
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Overview

authors

  • Luo, Y.
  • Knuckley, B.
  • Bhatia, M.
  • Pellechia, P. J.
  • Thompson, Paul

publication date

  • 2006

journal

  • Journal of the American Chemical Society  Journal

abstract

  • Protein arginine deiminase 4 (PAD4), which catalyzes the post-translational conversion of peptidyl arginine to peptidyl citrulline, is widely regarded as one of the best new targets for the development of a novel rheumatoid arthritis therapeutic. In addition to its presumed role in this disease, PAD4 is also a calcium-dependent histone deiminase that acts as a transcriptional co-repressor. Herein we describe the design, synthesis, and in vitro evaluation of two fluorescently labeled activity-based protein profiling (ABPP) reagents that specifically and irreversibly modify the active, that is, calcium-bound, form PAD4 with equal affinity to previously described small molecule chemical probes of PAD4 function. These fluorescently tagged ABPPs will be useful for identifying the conditions under which this enzyme is activated in vivo and may prove to be useful RA diagnostics.

subject areas

  • Fluorescent Dyes
  • Hydrolases
  • In Vitro Techniques
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Identity

PubMed Central ID

  • PMC2234596

International Standard Serial Number (ISSN)

  • 0002-7863

Digital Object Identifier (DOI)

  • 10.1021/ja0656907

PubMed ID

  • 17090024
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Additional Document Info

start page

  • 14468

end page

  • 14469

volume

  • 128

issue

  • 45

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