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Monoclonal-antibody analysis of purified and cell-associated tissue factor

Academic Article
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Overview

authors

  • Morrissey, J. H.
  • Fair, D. S.
  • Edgington, Thomas

publication date

  • November 1988

journal

  • Thrombosis Research  Journal

abstract

  • Tissue factor (TF), one of the cell-surface initiators of blood coagulation, has been implicated as the major molecule of this type and as a critical controlling molecule in hemostasis, thrombosis and inflammation. Analysis of the expression of human TF by cells has been hampered by the lack of suitable molecular probes. We have prepared a library of twenty-four murine hybridomas which stably secrete monoclonal antibodies to human TF. Based on their characteristics, these monoclonals can be categorized into a minimum of five distinct groups. Twenty-three of the hybridoma antibodies strongly inhibited TF activity, which was attributable to blocking of formation of the bimolecular complex of TF and factor VII. We have used these antibodies to demonstrate directly that TF is the sole high affinity factor VII receptor on an intact cell. We have also demonstrated the immunologic relationship between constitutive and induced expression of the protein responsible for TF-like activity by several cells and tissues. Most of the antibodies were found to inhibit TF activity expressed by other primate species, and the potential in vivo therapeutic use of monoclonal antibodies of differing intramolecular specificity is discussed.

subject areas

  • Animals
  • Antibodies, Monoclonal
  • Antigen-Antibody Reactions
  • Binding Sites, Antibody
  • Binding, Competitive
  • Blood Coagulation Tests
  • Cattle
  • Cell Line
  • Cercopithecus aethiops
  • Cross Reactions
  • Dogs
  • Factor VII
  • Female
  • Humans
  • Mice
  • Mice, Inbred BALB C
  • Molecular Probes
  • Papio
  • Rabbits
  • Rats
  • Thromboplastin
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Identity

International Standard Serial Number (ISSN)

  • 0049-3848

Digital Object Identifier (DOI)

  • 10.1016/0049-3848(88)90084-9

PubMed ID

  • 3194899
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Additional Document Info

start page

  • 247

end page

  • 261

volume

  • 52

issue

  • 3

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