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A mutagenesis study of a catalytic antibody

Academic Article
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Overview

authors

  • Jackson, D. Y.
  • Prudent, J. R.
  • Baldwin, E. P.
  • Schultz, Peter

publication date

  • January 1991

journal

  • Proceedings of the National Academy of Sciences of the United States of America  Journal

abstract

  • We have generated seven site-specific mutations in the genes encoding the variable region of the heavy chain domain (VH) of the phosphocholine-binding antibody S107. S107 is a member of a family of well-characterized highly homologous antibodies that bind phosphorylcholine mono- and diesters. Two of these antibodies, MOPC-167 and T15, have previously been shown to catalyze the hydrolysis of 4-nitrophenyl N-trimethylammonioethyl carbonate. Two conserved heavy-chain residues, Tyr-33 and Arg-52, were postulated to be involved in binding and hydrolysis of 4-nitrophenylcholine carbonate esters. To more precisely define the catalytic roles of these residues, three Arg-52 mutants (R52K, R52Q, R52C) and four Tyr-33 mutants (Y33H, Y33F, Y33E, Y33D) of antibody S107 were generated. The genes encoding the VH binding domain of S107 were inserted into plasmid pUC-fl, and in vitro mutagenesis was performed. The wild-type and mutant S107 antibodies were expressed in P-3X63-Ag8.653 (P-3) myeloma cells by using a modified SV2 shuttle vector. The catalytic properties of wild-type antibody S107 are similar to those of the phosphocholine-specific antibody T15, which has the same VH protein sequence. In general, mutations at Tyr-33 had little effect on catalytic activity, whereas mutations at Arg-52 that result in loss of the positively charged side chain significantly lower the catalytic activity of S107. One mutant, Y33H, catalyzed the hydrolysis of 4-nitrophenyl N-trimethylammonioethyl carbonate with a kcat of 5.7 min-1 and a Km of 1.6 mM at pH 7.5. These results not only demonstrate the importance of electrostatic interactions in catalysis by antibody S107 but also show that catalytic side chains can be introduced into antibodies to enhance their catalytic efficiency.

subject areas

  • Animals
  • Antibodies
  • Antibodies, Monoclonal
  • Cell Line
  • Enzyme-Linked Immunosorbent Assay
  • Genetic Vectors
  • Immunoglobulin Heavy Chains
  • Immunoglobulin Variable Region
  • Mutagenesis, Site-Directed
  • Plasmids
  • Restriction Mapping
  • Transfection
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Research

keywords

  • ANTIBODY CATALYSIS
  • MUTAGENESIS
  • PHOSPHOCHOLINE
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Identity

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.88.1.58

PubMed ID

  • 1898778
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Additional Document Info

start page

  • 58

end page

  • 62

volume

  • 88

issue

  • 1

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