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Identification of amino-acid-residues associated with the 2fe-2s cluster of the 25-kda (nqo2) subunit of the proton-translocating nadh-quinone oxidoreductase of paracoccus-denitrificans

Academic Article
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Overview

authors

  • Yano, T.
  • Sled, V. D.
  • Ohnishi, T.
  • Yagi, Takao

publication date

  • November 1994

journal

  • FEBS Letters  Journal

abstract

  • In order to identify the ligand residues of the [2Fe-2S] cluster of the 25 kDa (NQO2) subunit of the proton-translocating NADH-quinone oxidoreductase of Paracoccus denitrificans, we mutated individually all seven cysteine residues (C61, C96, C101, C104, C113, C137, and C141) and one conserved histidine residue (H92) to Ser or Ala and expressed them in E. coli. After purification of the mutated 25 kDa subunits, the effect of mutations on the iron-sulfur cluster were characterized by chemical analyses and UV-visible and EPR spectroscopy. All mutated subunits, especially mutants of conserved cysteines, contained lower amounts of non-heme iron than wild-type. The subunits of three non-conserved cysteine residues (C61, C104, and C113) mutated to Ser and a histidine residue (H92) mutated to Ala exhibited essentially the same spectroscopic properties as those of the wild-type subunit. In contrast, mutation of the four conserved cysteine residues (C96, C101, C137, and C141) to Ser or Ala considerably altered the UV-visible and EPR spectra from the wild-type subunit. These results indicate that the four conserved cysteine residues coordinate the [2Fe-2S] cluster in the P. denitrificans 25 kDa subunit.

subject areas

  • Amino Acids
  • Base Sequence
  • Biological Transport
  • Cysteine
  • Electron Spin Resonance Spectroscopy
  • Escherichia coli
  • Histidine
  • Iron-Sulfur Proteins
  • Metalloproteins
  • Molecular Sequence Data
  • Molecular Weight
  • Mutagenesis, Site-Directed
  • Nonheme Iron Proteins
  • Paracoccus denitrificans
  • Protons
  • Quinone Reductases
  • Spectrophotometry, Ultraviolet
  • Structure-Activity Relationship
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Research

keywords

  • EXPRESSION
  • IRON-SULFUR CLUSTER
  • NADH-QUINONE OXIDOREDUCTASE
  • PARACOCCUS DENITRIFICANS
  • SITE-DIRECTED MUTATION
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Identity

International Standard Serial Number (ISSN)

  • 0014-5793

Digital Object Identifier (DOI)

  • 10.1016/0014-5793(94)01107-9

PubMed ID

  • 7957917
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Additional Document Info

start page

  • 160

end page

  • 164

volume

  • 354

issue

  • 2

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