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Generation of a hybrid sequence-specific single-stranded deoxyribonuclease

Academic Article
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Overview

authors

  • Corey, D. R.
  • Schultz, Peter

publication date

  • December 1987

journal

  • Science  Journal

abstract

  • The relatively nonspecific single-stranded deoxyribonuclease, staphylococcal nuclease, was selectively fused to an oligonucleotide binding site of defined sequence to generate a hybrid enzyme. A cysteine was substituted for Lys116 in the enzyme by oligonucleotide-directed mutagenesis and coupled to an oligonucleotide that contained a 3'-thiol. The resulting hybrid enzyme cleaved single-stranded DNA at sites adjacent to the oligonucleotide binding site.

subject areas

  • Amino Acid Sequence
  • Base Sequence
  • DNA, Single-Stranded
  • Hydrolysis
  • Micrococcal Nuclease
  • Models, Molecular
  • Mutation
  • Protein Conformation
  • Staphylococcus aureus
  • Substrate Specificity
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Identity

International Standard Serial Number (ISSN)

  • 0036-8075

Digital Object Identifier (DOI)

  • 10.1126/science.3685986

PubMed ID

  • 3685986
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Additional Document Info

start page

  • 1401

end page

  • 1403

volume

  • 238

issue

  • 4832

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