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The t-cell receptor v-alpha-11 gene family - analysis of allelic sequence polymorphism and demonstration of j-alpha region-dependent recognition by allele-specific antibodies

Academic Article
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Overview

authors

  • Jameson, S. C.
  • Nakajima, P. B.
  • Brooks, J. L.
  • Heath, W.
  • Kanagawa, O.
  • Gascoigne, Nicholas

publication date

  • 1991

journal

  • Journal of Immunology  Journal

abstract

  • Allelic polymorphism in TCR loci may play an important role in shaping the T cell repertoire and in disease susceptibility. We have used a combination of antibody and sequence analysis to investigate polymorphism in the murine V alpha 11 family. Two different antibodies have been analyzed that recognize particular V alpha 11 family members of the V alpha b and V alpha d haplotypes. One antibody shows J alpha dependency, suggesting a conformational element to the epitope. Investigation of the anti-V alpha 11 staining pattern on different mouse strains indicates that there is a marked influence of MHC haplotype on V alpha 11 selection and that V alpha 11 is preferentially expressed on CD4+ cells. Sequence analysis of V alpha 11 genes from the V alpha a, V alpha b, and V alpha d haplotypes shows two potential regions for the haplotype-specific epitopes. The relatedness of the different V alpha 11 family members from different haplotypes suggests that the V alpha 11.1/11.2 gene duplication is relatively recent, but that V alpha 11.3 separated much earlier. Differences between V alpha 11.3 and V alpha 11.1/11.2 are concentrated in the putative complementarity determining regions (CDR), whereas differences between alleles are not clearly clustered. However, the V alpha 11.1a and V alpha 11.1d alleles differ from V alpha 11.1b and V alpha 11.2b in CDR1. A V alpha 11.2-expressing anti-cytochrome c T cell has the same V-J junction as a V alpha 11.1-bearing cell with a similar fine specificity, indicating that V alpha 11.1b and V alpha 11.2b do not contribute different Ag specificities.

subject areas

  • Alleles
  • Amino Acid Sequence
  • Animals
  • Antibodies, Monoclonal
  • Base Sequence
  • Cytochrome c Group
  • Epitopes
  • Gene Rearrangement, alpha-Chain T-Cell Antigen Receptor
  • Isoantibodies
  • Mice
  • Mice, Inbred Strains
  • Molecular Sequence Data
  • Oligonucleotides
  • Polymorphism, Genetic
  • Radiation Chimera
  • Receptors, Antigen, T-Cell, alpha-beta
  • T-Lymphocytes
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Identity

International Standard Serial Number (ISSN)

  • 0022-1767

PubMed ID

  • 1717591
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Additional Document Info

start page

  • 3185

end page

  • 3193

volume

  • 147

issue

  • 9

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