Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form

Characterization of endogenous and recombinant proviral elements of a highly tumorigenic akr cell-line

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Lamont, C.
  • Culp, P.
  • Talbott, R. L.
  • Phillips, T. R.
  • Trauger, R. J.
  • Frankel, W. N.
  • Wilson, M. C.
  • Coffin, J. M.
  • Elder, John

publication date

  • September 1991

journal

  • Journal of Virology  Journal

abstract

  • As an approach to evaluating the contribution of classes of endogenous viral sequences to leukemogenesis, a genomic library was prepared from the highly tumorigenic AKR SL12.3 cell line and screened for env-containing proviruses. An extensive battery of virus-derived probes and specific oligonucleotide probes were used to segregate 83 positive clones into related groups. The nonecotropic endogenous retroviruses were identified as members of the polytropic, modified polytropic, or xenotropic groups. At least three unique xenotropic proviruses were detected that differed from the published xenotropic sequence within a variable region of the 5' portion of env. Changes among the xenotropic proviruses included relative insertions and/or deletions that maintain an open reading frame and hence the potential to encode viable envelope gene products. Several recombinant viruses were also detected. Recombination was not random and primarily involved the formation of mink cell focus-inducing class I retroviruses via recombination between polytropic elements and ecotropic virus. One other recombinant was detected which contained ecotropic virus sequences in the 5' region encoding p15 of an otherwise xenotropic provirus. An interesting observation was the finding that certain clones contained more than one provirus within the average 20-kb cloned insert. This would not be expected if integration were totally random. The de novo recombinant proviruses identified here provide a series of potential candidates to be evaluated for their contribution to the tumorigencity of the SL12.3 cell line.

subject areas

  • Base Sequence
  • Blotting, Southern
  • Cell Transformation, Viral
  • DNA, Viral
  • Gene Products, env
  • Genomic Library
  • Molecular Sequence Data
  • Oncogenes
  • Recombination, Genetic
  • Retroviridae
  • Species Specificity
  • Tumor Cells, Cultured
  • Virus Replication
scroll to property group menus

Identity

PubMed Central ID

  • PMC248916

International Standard Serial Number (ISSN)

  • 0022-538X

PubMed ID

  • 1870192
scroll to property group menus

Additional Document Info

start page

  • 4619

end page

  • 4628

volume

  • 65

issue

  • 9

©2021 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support