Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form

Activity-based probes that target functional subclasses of phospholipases in proteomes

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Tully, S. E.
  • Cravatt, Benjamin

publication date

  • March 2010

journal

  • Journal of the American Chemical Society  Journal

abstract

  • Phospholipases are a large and diverse set of enzymes that metabolize the phospholipid components of cell membranes and function in key lipid-signaling pathways. The molecular characterization of novel phospholipases would benefit from chemical probes that selectively target these enzymes on the basis of their distinct substrate specificities and catalytic properties. Here we present the synthesis and characterization of a set of activity-based protein profiling (ABPP) probes that contain key recognition and reactivity elements for targeting phospholipases of the serine hydrolase superfamily. We show that these probes accurately report on the sn-1 and sn-2 substrate specificities of phospholipases in cell and tissue proteomes, including the sn-1-selective phospholipase DDHD1 and a calcium-dependent transacylase activity implicated in endocannabinoid biosynthesis. We anticipate that these phospholipase-directed ABPP probes will facilitate the discovery of new lipid-metabolizing enzymes and provide valuable insights into their substrate preferences.

subject areas

  • Alkynes
  • Animals
  • Mice
  • Organophosphonates
  • Phospholipases
  • Proteome
  • Rats
  • Substrate Specificity
scroll to property group menus

Identity

PubMed Central ID

  • PMC2844343

International Standard Serial Number (ISSN)

  • 0002-7863

Digital Object Identifier (DOI)

  • 10.1021/ja1000505

PubMed ID

  • 20178358
scroll to property group menus

Additional Document Info

start page

  • 3264

end page

  • 3265

volume

  • 132

issue

  • 10

©2021 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support