Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form
As of April 1st VIVO Scientific Profiles will no longer updated for faculty, and the link to VIVO will be removed from the library website. Faculty profile pages will continue to be updated via Interfolio. VIVO will continue being used behind the scenes to update graduate student profiles. Please contact helplib@scripps.edu if you have questions.
How to download citations from VIVO | Alternative profile options

Masking of cd22 by cis ligands does not prevent redistribution of cd22 to sites of cell contact

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Collins, B. E.
  • Blixt, O.
  • DeSieno, A. R.
  • Bovin, N.
  • Marth, J. D.
  • Paulson, James

publication date

  • April 2004

journal

  • Proceedings of the National Academy of Sciences of the United States of America  Journal

abstract

  • CD22, a negative regulator of B cell signaling, is a member of the siglec family that binds to alpha2-6-linked sialic acids on glycoproteins. Previous reports demonstrated that binding of multivalent sialoside probes to CD22 is blocked, or "masked," by endogenous (cis) ligands, unless they are first destroyed by sialidase treatment. These results suggest that cis ligands on B cells make CD22 functionally unavailable for binding to ligands in trans. Through immunofluorescence microscopy, however, we observed that CD22 on resting B cells redistributes to the site of contact with other B or T lymphocytes. Redistribution is mediated by interaction with trans ligands on the opposing cell because it does not occur with ligand-deficient lymphocytes from ST6GalI-null mice. Surprisingly, CD45, proposed as both a cis and trans ligand of CD22, was not required for redistribution to sites of cell contact, given that redistribution of CD22 was independent of CD45 and was observed with lymphocytes from CD45-deficient mice. Furthermore, CD45 is not required for CD22 masking as similar levels of masking were observed in the WT and null mice. Comparison of the widely used sialoside-polyacrylamide probe with a sialoside-streptavidin probe revealed that the latter bound a subset of B cells without sialidase treatment, suggesting that cis ligands differentially impacted the binding of these two probes in trans. The combined results suggest that equilibrium binding to cis ligands does not preclude binding of CD22 to ligands in trans, and allows for its redistribution to sites of contact between lymphocytes.

subject areas

  • Animals
  • Antigens, CD
  • Antigens, Differentiation, B-Lymphocyte
  • Cell Adhesion Molecules
  • Flow Cytometry
  • Lectins
  • Ligands
  • Mice
  • Mice, Inbred C57BL
  • Microscopy, Fluorescence
  • Molecular Probes
  • Sialic Acid Binding Ig-like Lectin 2
scroll to property group menus

Identity

PubMed Central ID

  • PMC395930

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.0400851101

PubMed ID

  • 15079087
scroll to property group menus

Additional Document Info

start page

  • 6104

end page

  • 6109

volume

  • 101

issue

  • 16

©2022 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support