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Assessing enzyme activities using stable isotope labeling and mass spectrometry

Academic Article
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Overview

authors

  • Everley, P. A.
  • Gartner, C. A.
  • Haas, W.
  • Saghatelian, Alan
  • Elias, J. E.
  • Cravatt, Benjamin
  • Zetter, B. R.
  • Gygi, S. P.

publication date

  • 2007

journal

  • Molecular & Cellular Proteomics  Journal

abstract

  • Activity-based protein profiling has emerged as a valuable technology for labeling, enriching, and assessing protein activities from complex mixtures. This is primarily accomplished via a two-step identification and quantification process. Here we show a highly quantitative and streamlined method, termed catch-and-release activity profiling of enzymes (CAPE), which reduces this procedure to a single step. Furthermore the CAPE approach has the ability to detect small quantitative changes that may have been missed by alternative mass spectrometry-based techniques.

subject areas

  • Amino Acid Sequence
  • Animals
  • Cattle
  • Cell Line, Tumor
  • Humans
  • Isotope Labeling
  • Mass Spectrometry
  • Molecular Sequence Data
  • Neoplasm Metastasis
  • Neoplasm Proteins
  • Organophosphonates
  • Peptides
  • Trypsin
  • Trypsinogen
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Identity

International Standard Serial Number (ISSN)

  • 1535-9476

Digital Object Identifier (DOI)

  • 10.1074/mcp.M700057-MCP200

PubMed ID

  • 17627935
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Additional Document Info

start page

  • 1771

end page

  • 1777

volume

  • 6

issue

  • 10

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