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New Tetrohymena basal body protein components identify basal body domain structure

Academic Article
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Overview

authors

  • Kilburn, C. L.
  • Pearsorl, C. G.
  • Romiin, E. P.
  • Meehl, J. B.
  • Giddings, T. H.
  • Culver, B. P.
  • Yates III, John
  • Winey, M.

publication date

  • September 2007

journal

  • Journal of Cell Biology  Journal

abstract

  • Basal bodies organize the nine doublet microtubules found in cilia. Cilia are required for a variety of cellular functions, including motility and sensing stimuli. Understanding this biochemically complex organelle requires an inventory of the molecular components and the contribution each makes to the overall structure. We define a basal body proteome and determine the specific localization of basal body components in the ciliated protozoan Tetrahymena thermophila. Using a biochemical, bioinformatic, and genetic approach, we identify 97 known and candidate basal body proteins. 24 novel T. thermophila basal body proteins were identified, 19 of which were localized to the ultrastructural level, as seen by immunoelectron microscopy. Importantly, we find proteins from several structural domains within the basal body, allowing us to reveal how each component contributes to the overall organization. Thus, we present a high resolution localization map of basal body structure highlighting important new components for future functional studies.

subject areas

  • Animals
  • Centrioles
  • Cilia
  • Microscopy, Electron, Transmission
  • Microtubules
  • Proteome
  • Protozoan Proteins
  • Tetrahymena thermophila
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Identity

PubMed Central ID

  • PMC2064616

International Standard Serial Number (ISSN)

  • 0021-9525

Digital Object Identifier (DOI)

  • 10.1083/jcb.200703109

PubMed ID

  • 17785518
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Additional Document Info

start page

  • 905

end page

  • 912

volume

  • 178

issue

  • 6

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