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C-D modes of deuterated side chain of leucine as structural reporters via dual-frequency two-dimensional infrared spectroscopy

Academic Article
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Overview

related to degree

  • Thielges, Megan, Ph.D. in Biophysics, Scripps Research 2003 - 2009

authors

  • Naraharisetty, S. R. G.
  • Kasyanenko, V. M.
  • Zimmermann, J.
  • Thielges, Megan
  • Romesberg, Floyd
  • Rubtsov, I. V.

publication date

  • April 2009

journal

  • Journal of Physical Chemistry B  Journal

abstract

  • Perdeuteration of the side chains of amino acids such as leucine results in appearance of reasonably strong absorption peaks around 2050-2220 cm(-1) that belong to the CD stretching modes and exhibit extinction coefficients of up to 120 M(-1) cm(-1). The properties of the CD stretching transitions in leucine-d(10) as structural labels are studied via the methods of two-dimensional infrared (2DIR) spectroscopy. The cross peaks caused by interactions of the CD stretching modes with amide I (Am-I), CO, and amide II (Am-II) modes are obtained by the dual-frequency 2DIR method. The CD stretching peaks in leucine-d(10) are characterized using DFT computational modeling as well as relaxation-assisted 2DIR (RA 2DIR) measurements. The RA 2DIR measurements showed different enhancements and different energy transport times (arrival times) for different CD/Am-II and CD/CO cross peaks; a correlation between the intermode distance, the arrival time, and the amplification factor is reported. We demonstrated that the CD transitions of leucine-d(10) amino acid can serve as convenient structural reporters via the dual-frequency 2DIR methods and discussed the potential of leucine-d(10) and other amino acids with deuterium-labeled side chains as probes of protein structure and dynamics.

subject areas

  • Anisotropy
  • Computer Simulation
  • Energy Transfer
  • Leucine
  • Models, Chemical
  • Molecular Structure
  • Proteins
  • Spectrophotometry, Infrared
  • Time Factors
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Identity

International Standard Serial Number (ISSN)

  • 1520-6106

Digital Object Identifier (DOI)

  • 10.1021/jp8112446

PubMed ID

  • 19298041
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Additional Document Info

start page

  • 4940

end page

  • 4946

volume

  • 113

issue

  • 14

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