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Silver staining of nucleic-acids - applications in virus research and in diagnostic virology

Academic Article
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Overview

authors

  • Whitton, J. Lindsay
  • Hundley, F.
  • Odonnell, B.
  • Desselberger, U.

publication date

  • 1983

journal

  • Journal of Virological Methods  Journal

abstract

  • The rapid and sensitive method of silver staining of nucleic acids in polyacrylamide gels is used to visualise double-stranded (ds) and single-stranded (ss) molecules of both DNA and RNA. The binding of silver ions to nucleic acids in gels is stoichiometric and independent of the GC content of ds DNA. Silver staining can be applied to problems in virus research and to the analysis of clinical virus isolates.

subject areas

  • DNA, Viral
  • Electrophoresis, Polyacrylamide Gel
  • Humans
  • Influenza, Human
  • Methods
  • RNA, Double-Stranded
  • RNA, Viral
  • Retroviridae Infections
  • Silver
  • Staining and Labeling
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Identity

International Standard Serial Number (ISSN)

  • 0166-0934

Digital Object Identifier (DOI)

  • 10.1016/0166-0934(83)90008-3

PubMed ID

  • 6197422
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Additional Document Info

start page

  • 185

end page

  • 198

volume

  • 7

issue

  • 4

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