Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form
As of April 1st VIVO Scientific Profiles will no longer updated for faculty, and the link to VIVO will be removed from the library website. Faculty profile pages will continue to be updated via Interfolio. VIVO will continue being used behind the scenes to update graduate student profiles. Please contact helplib@scripps.edu if you have questions.
How to download citations from VIVO | Alternative profile options

Analysis of individual immunoglobulin-lambda light-chain genes amplified from single cells is inconsistent with variable region gene conversion in germinal-center b-cell somatic mutation

Academic Article
uri icon
  • Overview
  • Research
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Ford, J. E.
  • McHeyzer-Williams, Michael G.
  • Lieber, M. R.

publication date

  • August 1994

journal

  • European Journal of Immunology  Journal

abstract

  • Responding B cells in specific immune responses diversify their immunoglobulin genes and are selected on their variant antigen receptors in the microenvironment of the germinal center. The patterns of mutations previously reported for immunglobulin (Ig) genes have supported mechanistic hypotheses of either error-prone DNA synthesis or templated variable region gene conversion as the underlying mechanism in the generation of these mutations. To assess the role of gene conversion in germinal-center somatic mutation, we chose to examine nucleotide changes in mouse lambda light chain genes which arose in response to a specific antigen. Laboratory mice possess three V lambda subexons, two of which differ from one another by only seven nucleotides, making these two subexons ideal for gene conversion. In the current study, we used six-parameter flow cytometry to isolate single lambda light chain-expressing germinal-center B cells from two different time points in a primary immune response. We then individually amplified and sequenced individual V lambda 1 genes from these single cells for mutational analysis. None of the 32 V lambda 1 genes, containing a total of 40 mutations, showed evidence of gene conversion from either of the other V lambda subexons. Features such as the replacement to silent ratio of the mutations documented at the earlier time point indicate an absence of antigen-driven selection. These data indicate that V region gene conversion does not contribute to germinal-center somatic mutation and that gene conversion is not responsible for targeting mutation specifically to rearranged Ig genes. The biological implications are discussed.

subject areas

  • Amino Acid Sequence
  • Animals
  • B-Lymphocytes
  • Base Sequence
  • Flow Cytometry
  • Gene Rearrangement, B-Lymphocyte
  • Immunoglobulin Variable Region
  • Immunoglobulin lambda-Chains
  • Lymph Nodes
  • Mice
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Mutation
scroll to property group menus

Research

keywords

  • GENE CONVERSION
  • LAMBDA LIGHT CHAIN
  • SOMATIC MUTATION
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 0014-2980

Digital Object Identifier (DOI)

  • 10.1002/eji.1830240814

PubMed ID

  • 8056040
scroll to property group menus

Additional Document Info

start page

  • 1816

end page

  • 1822

volume

  • 24

issue

  • 8

©2022 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support