Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form

Blockade of galanin-induced inhibition of insulin secretion from isolated mouse islets by the non-methionine containing antagonist M35

Academic Article
uri icon
  • Overview
  • Research
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Gregersen, S.
  • Lindskog, S.
  • Land, T.
  • Langel, Ülo
  • Bartfai, Tamas
  • Ahren, B.

publication date

  • 1993

journal

  • European Journal of Pharmacology  Journal

abstract

  • The neuropeptide galanin occurs in pancreatic adrenergic nerves and has been suggested to be the adrenergic mediator of the stress-induced inhibition of insulin release. To study its physiological function, we recently synthesized a galanin-like galanin receptor antagonist, galantide. However, this antagonist contains a methionine moiety, and is therefore easily oxidized. We have now synthesized another galanin antagonist which does not contain methionine. This peptide, M35, is a chimeric 21 amino acid peptide in which galanin-(1-13) is coupled to bradykinin-(2-9). M35 (10 microM to 1 pM) had no effect by itself on glucose (11.1 mM)-stimulated insulin secretion in isolated mouse islets, but potently counteracted the inhibitory action of galanin (100 nM). The lowest effective dose of M35 was 10 nM. M35 did not counteract the inhibitory action of clonidine (1 microM) or somatostatin (1 microM) on insulin secretion. Furthermore, M35 displaced 125I-monoiodo-[Tyr26]galanin from membranes of insulin producing RINm5F cells. The displacement curve fitted to a two-site model in which 60% of label bound with a K1 of 0.1 +/- 0.01 nM and 40% with a K2 of 3 +/- 0.5 nM. In conclusion, M35 is a specific, non-methionine-containing galanin receptor antagonist on insulin-producing cells.

subject areas

  • Amino Acid Sequence
  • Animals
  • Binding, Competitive
  • Bradykinin
  • Clonidine
  • Galanin
  • In Vitro Techniques
  • Insulin
  • Islets of Langerhans
  • Mice
  • Mice, Inbred Strains
  • Molecular Sequence Data
  • Neuropeptides
  • Peptide Fragments
  • Peptides
  • Somatostatin
scroll to property group menus

Research

keywords

  • CLONIDINE
  • GALANIN
  • INSULIN SECRETION
  • ISLETS OF LANGERHANS (ISOLATED)
  • M35
  • SOMATOSTATIN
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 0014-2999

Digital Object Identifier (DOI)

  • 10.1016/0014-2999(93)90725-w

PubMed ID

  • 7681407
scroll to property group menus

Additional Document Info

start page

  • 35

end page

  • 39

volume

  • 232

issue

  • 1

©2019 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support