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Adult chicken alpha-globin gene-expression in transfected qt6 quail cells - evidence for a negative regulatory element in the alpha-d gene region

Academic Article
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Overview

authors

  • Lewis, W.
  • Lee, Jiing-Dwan
  • Dodgson, J. B.

publication date

  • October 1991

journal

  • Nucleic Acids Research  Journal

abstract

  • The chicken adult alpha-globin genes, alpha A and alpha D, are closely linked in chromosomal DNA and are coordinately expressed in vivo in an approximate 3:1 ratio, respectively. When subcloned DNAs containing one or the other gene are stably transfected into QT6 quail fibroblasts, the alpha A-globin gene is expressed at measurable RNA levels, but the alpha D gene is not. The alpha A gene expression can be considerably increased by the presence of a linked Rous sarcoma virus long terminal repeat enhancer, but that of the alpha D gene remains undetectable. Transfection with subclones containing both genes, either in cis or in trans, leads to considerably reduced alpha A RNA levels and still no observable alpha D gene expression. Transfection with deleted subclones suggests that maximal expression levels in this system require the alpha A-globin gene promoter, as opposed to that of the alpha D gene, but that such expression is greatly reduced by one or more DNA sequences which lie approximately 2,000 base pairs upstream of the alpha A gene, within the body of the alpha D gene.

subject areas

  • Animals
  • Avian Sarcoma Viruses
  • Base Sequence
  • Cell Line
  • Chickens
  • Cloning, Molecular
  • Enhancer Elements, Genetic
  • Gene Expression Regulation
  • Globins
  • HeLa Cells
  • Humans
  • Molecular Sequence Data
  • Multigene Family
  • Mutation
  • Promoter Regions, Genetic
  • Regulatory Sequences, Nucleic Acid
  • Repetitive Sequences, Nucleic Acid
  • Restriction Mapping
  • Transfection
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Identity

International Standard Serial Number (ISSN)

  • 0305-1048

Digital Object Identifier (DOI)

  • 10.1093/nar/19.19.5321

PubMed ID

  • 1656392
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Additional Document Info

start page

  • 5321

end page

  • 5329

volume

  • 19

issue

  • 19

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