Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form

Analysis of effector-cells in human antibody-dependent cellular cytotoxicity with murine monoclonal-antibodies

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Ortaldo, J. R.
  • Woodhouse, C.
  • Morgan, A. C.
  • Herberman, R. B.
  • Cheresh, D. A.
  • Reisfeld, Ralph

publication date

  • 1987

journal

  • Journal of Immunology  Journal

abstract

  • We examined purified human large granular lymphocytes, peripheral monocytes, and T cells for their ability to mediate antibody-dependent cellular cytotoxicity (ADCC) with murine monoclonal antibodies. We also evaluated the effects of pretreatment of cells with interleukin 2 and interferon to augment ADCC activity. MB3.6, a murine monoclonal antibody directed against the GD3 ganglioside, induced high levels of ADCC. This ADCC was mediated predominantly, if not completely, by human killer cells (large granular lymphocytes) whereas other effector cell populations demonstrated no significant cytotoxic activity in 6- or 18-hr assays. The IgG2a an anti-melanoma antibody 9.2.27 generated low or no ADCC with most normal donors or melanoma patients. IL 2 was a very potent booster of ADCC activity. Interferon alpha also was effective, whereas interferon gamma did not augment but rather inhibited reactivity. We tested a large panel of antibodies of various isotype against colon carcinoma cells and found that gamma-3 isotype antibodies more frequently generated ADCC and produced higher levels of cytotoxic activity than did IgG1 or IgG2 antibodies. It appears that a variety of parameters can affect ADCC reactions, including the type of effector cell and its level of activation, the isotype of the antibody, and properties of the target cell line such as its susceptibility to lysis.

subject areas

  • Animals
  • Antibodies, Monoclonal
  • Antibodies, Neoplasm
  • Antibody-Dependent Cell Cytotoxicity
  • Colonic Neoplasms
  • Cytotoxicity Tests, Immunologic
  • Gangliosides
  • Humans
  • Immunoglobulin G
  • Immunoglobulin Isotypes
  • Interferons
  • Interleukin-2
  • Killer Cells, Natural
  • Melanoma
  • Mice
  • Monocytes
  • T-Lymphocytes
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 0022-1767

PubMed ID

  • 2437198
scroll to property group menus

Additional Document Info

start page

  • 3566

end page

  • 3572

volume

  • 138

issue

  • 10

©2019 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support