related to degree

• Iacovoni, Jason Scott, Ph.D. in Biology, Scripps Research 1993 - 2003

authors

• Goller, M. E.
• Iacovoni, Jason Scott
• Vogt, Peter K.
• Kruse, U.

publication date

• 1998

journal

• Oncogene  Journal

abstract

• We have analysed differential gene expression in v-jun-transformed chicken embryo fibroblasts (CEF) compared to normal CEF by using the directional tag PCR subtraction method. From a first generation of putative Jun targets four clones were selected for study; they are upregulated in jun-transformed cells. Three of these clones showed homology to known genes: glutaredoxin, growth associated protein (GAP)-43/neuromodulin, and phenobarbital-induced cytochrome P450. The expression of these genes was analysed in fibroblasts transformed by various oncogenes. Expression of the glutaredoxin mRNA could be induced by a Jun-estrogen receptor chimaera in the absence of de novo protein biosynthesis. Based on this observation we conclude that glutaredoxin is a direct target of v-Jun.

subject areas

• Animals
• Chick Embryo
• GAP-43 Protein
• Gene Expression Regulation
• Glutaredoxins
• Oncogene Protein p65(gag-jun)
• Oxidoreductases
• Proteins

keywords

• cytochrome P450
• differential expression
• glutaredoxin
• growth associated protein (GAP)-43
• jun
• subtractive hybridization
• target gene

International Standard Serial Number (ISSN)

• 0950-9232

Digital Object Identifier (DOI)

• 10.1038/sj.onc.1201819

PubMed ID

• 9671415

start page

• 2945

end page

• 2948

volume

• 16

issue

• 22