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In vivo-activated cd4 t cells upregulate cxc chemokine receptor 5 and reprogram their response to lymphoid chemokines

Academic Article
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Overview

authors

  • Ansel, K. M.
  • McHeyzer-Williams, L. J.
  • Ngo, V. N.
  • McHeyzer-Williams, Michael G.
  • Cyster, J. G.

publication date

  • 1999

journal

  • Journal of Experimental Medicine  Journal

abstract

  • Migration of antigen-activated CD4 T cells to B cell areas of lymphoid tissues is important for mounting T cell-dependent antibody responses. Here we show that CXC chemokine receptor (CXCR)5, the receptor for B lymphocyte chemoattractant (BLC), is upregulated on antigen-specific CD4 T cells in vivo when animals are immunized under conditions that promote T cell migration to follicles. In situ hybridization of secondary follicles for BLC showed high expression in mantle zones and low expression in germinal centers. When tested directly ex vivo, CXCR5(hi) T cells exhibited a vigorous chemotactic response to BLC. At the same time, the CXCR5(hi) cells showed reduced responsiveness to the T zone chemokines, Epstein-Barr virus-induced molecule 1 (EBI-1) ligand chemokine (ELC) and secondary lymphoid tissue chemokine (SLC). After adoptive transfer, CXCR5(hi) CD4 T cells did not migrate to follicles, indicating that additional changes may occur after immunization that help direct T cells to follicles. To further explore whether T cells could acquire an intrinsic ability to migrate to follicles, CD4(-)CD8(-) double negative (DN) T cells from MRL-lpr mice were studied. These T cells normally accumulate within follicles of MRL-lpr mice. Upon transfer to wild-type recipients, DN T cells migrated to follicle proximal regions in all secondary lymphoid tissues. Taken together, our findings indicate that reprogramming of responsiveness to constitutively expressed lymphoid tissue chemokines plays an important role in T cell migration to the B cell compartment of lymphoid tissues.

subject areas

  • Animals
  • Antigens, CD8
  • CD4-Positive T-Lymphocytes
  • Cell Movement
  • Chemokine CCL19
  • Chemokine CCL21
  • Chemokine CXCL13
  • Chemokines, CC
  • Chemokines, CXC
  • Flow Cytometry
  • Fluorescent Antibody Technique
  • Germinal Center
  • In Situ Hybridization, Fluorescence
  • Lymph Nodes
  • Lymphocyte Activation
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred MRL lpr
  • Receptors, CXCR5
  • Receptors, Chemokine
  • Receptors, Cytokine
  • Spleen
  • Up-Regulation
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Research

keywords

  • CXCR5
  • ELC
  • T lymphocyte
  • chemokine
  • follicle
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Identity

International Standard Serial Number (ISSN)

  • 0022-1007

Digital Object Identifier (DOI)

  • 10.1084/jem.190.8.1123

PubMed ID

  • 10523610
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Additional Document Info

start page

  • 1123

end page

  • 1134

volume

  • 190

issue

  • 8

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