Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form

Multiple autoantigen binding capabilities of mouse monoclonal antibodies selected for rheumatoid factor activity

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Rubin, R. L.
  • Balderas, R. S.
  • Tan, Eng
  • Dixon, F. J.
  • Theofilopoulos, Argyrios

publication date

  • 1984

journal

  • Journal of Experimental Medicine  Journal

abstract

  • We report that approximately 1/4 of monoclonal rheumatoid factors produced by hybridomas derived from fusions of spleen cells from MRL/lpr/lpr mice with systemic lupus erythematosus (SLE) and arthritis exhibited multiple reactivities with other autoantigens, including dDNA , histones, and/or cytoskeletal-cytoplasmic elements. The patterns of reactivities of most of these clones differed, indicating that each had a separate B cell ancestor. Studies with eluted antibodies demonstrated that a single species of antibody molecules was responsible for the observed multiple reactivities. Inhibition experiments suggested that an antibody combining site may be large enough to accommodate dissimilar epitopes. These findings may provide further insights into the generation and extent of antibody diversity as well as the etiopathogenesis of systemic autoimmune diseases.

subject areas

  • Animals
  • Antibodies, Monoclonal
  • Antigens
  • Autoantigens
  • Binding Sites, Antibody
  • Binding, Competitive
  • Cross Reactions
  • DNA, Single-Stranded
  • Fluorescent Antibody Technique
  • Histones
  • Hybridomas
  • Immunoglobulin G
  • Mice
  • Mice, Mutant Strains
  • Polynucleotides
  • Rheumatoid Factor
scroll to property group menus

Identity

PubMed Central ID

  • PMC2187302

International Standard Serial Number (ISSN)

  • 0022-1007

Digital Object Identifier (DOI)

  • 10.1084/jem.159.5.1429

PubMed ID

  • 6371180
scroll to property group menus

Additional Document Info

start page

  • 1429

end page

  • 1440

volume

  • 159

issue

  • 5

©2019 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support