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Heterochromatin protein HP1Hsbeta (p25beta) and its localization with centromeres in mitosis

Academic Article
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Overview

authors

  • Furuta, K.
  • Chan, E. K. L.
  • Kiyosawa, K.
  • Reimer, G.
  • Luderschmidt, C.
  • Tan, Eng

publication date

  • June 1997

journal

  • Chromosoma  Journal

abstract

  • Some autoimmune sera containing anticentromere autoantibodies also recognize a doublet of Mr 23 000 (p23) and 25 000 (p25) in addition to CENP (centromere protein)-A (Mr 19 000), -B (Mr 80 000), and -C (Mr 140 000). A p25 antigen (HP1(Hsalpha)) has been shown to be a human homolog of Drosophila HP1 (heterochromatin protein 1). We have isolated a cDNA clone encoding another form of p25 (HP1(Hsbeta ) or p25beta) from a lambdaZap HepG2 library using human autoimmune serum. The deduced amino acid sequence of the clone contained a conserved chromodomain (chromatin modifier domain) in the N-terminal region and a heterochromatin binding domain in the C-terminal region. In immunofluorescence experiments, only affinity purified antibodies reactive with the C-terminal (amino acids 70-185) domain showed nucleoplasmic and heterochromatin staining, whereas N-terminal (amino acids 1-115) specific antibodies were nonreactive. In metaphase chromosome spreads, the C-terminal domain antibody was also localized to the centromeric regions of chromosomes. Association with centromeres was most prominent at anaphase and changed to a more generalized association with whole chromosomes in telophase. The cooccurrence of autoantibodies to centromere proteins and HP1 in certain autoimmune diseases might be a reflection of coordinated immune responses to these closely associated sets of proteins.

subject areas

  • 3T3 Cells
  • Aged
  • Amino Acid Sequence
  • Animals
  • Autoantibodies
  • Base Sequence
  • Centromere
  • Chromatin
  • Chromosomal Proteins, Non-Histone
  • Chromosome Mapping
  • Cloning, Molecular
  • Cross Reactions
  • Drosophila
  • Female
  • Humans
  • Immune Sera
  • Mice
  • Mitosis
  • Molecular Sequence Data
  • Precipitin Tests
  • Sequence Analysis, DNA
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Identity

International Standard Serial Number (ISSN)

  • 0009-5915

Digital Object Identifier (DOI)

  • 10.1007/s004120050219

PubMed ID

  • 9169582
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Additional Document Info

start page

  • 11

end page

  • 19

volume

  • 106

issue

  • 1

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