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L-type calcium channels mediate calcium oscillations in early postnatal Purkinje neurons

Academic Article
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Overview

authors

  • Liljelund, P.
  • Netzeband, J. G.
  • Gruol, Donna

publication date

  • October 2000

journal

  • Journal of Neuroscience  Journal

abstract

  • Ca(2+) signaling is important in many fundamental neuronal processes including neurotransmission, synaptic plasticity, neuronal development, and gene expression. In cerebellar Purkinje neurons, Ca(2+) signaling has been studied primarily in the dendritic region where increases in local Ca(2+) have been shown to occur with both synaptic events and spontaneous electrical activity involving P-type voltage-gated Ca(2+) channels (VGCCs), the predominant VGCC expressed by Purkinje neurons. Here we show that Ca(2+) signaling is also a prominent feature of immature Purkinje neurons at developmental stages that precede expression of dendritic structure and involves L-type rather than P-type VGCCs. Immature Purkinje neurons acutely dissociated from postnatal day 4-7 rat pups exhibit spontaneous cytoplasmic Ca(2+) oscillations. The Ca(2+) oscillations require entry of extracellular Ca(2+), are blocked by tetrodotoxin, are communicated to the nucleus, and correlate closely with patterns of endogenously generated spontaneous and evoked electrical activity recorded in the neurons. Immunocytochemistry showed that L-, N-, and P/Q-types of VGCCs are present on the somata of the Purkinje neurons at this age. However, only the L-type VGCC antagonist nimodipine effectively antagonized the Ca(2+) oscillations; inhibitors of P/Q and N-type VGCCs were relatively ineffective. Release of Ca(2+) from intracellular Ca(2+) stores significantly amplified the Ca(2+) signals of external origin. These results show that a somatic signaling pathway that generates intracellular Ca(2+) oscillations and involves L-type VGCCs and intracellular Ca(2+) stores plays a prominent role in the Ca(2+) dynamics of early developing Purkinje neurons and may play an important role in communicating developmental cues to the nucleus.

subject areas

  • Action Potentials
  • Animals
  • Calcium
  • Calcium Channel Blockers
  • Calcium Channels, L-Type
  • Calcium Signaling
  • Cell Nucleus
  • Cells, Cultured
  • Cerebellum
  • Cytoplasm
  • Glial Fibrillary Acidic Protein
  • Patch-Clamp Techniques
  • Purkinje Cells
  • Rats
  • Sodium Channel Blockers
  • gamma-Aminobutyric Acid
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Research

keywords

  • Ca2+ signaling
  • acutely isolated neurons
  • cerebellum
  • development
  • intracellular Ca2+ stores
  • nuclear Ca2+
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Identity

International Standard Serial Number (ISSN)

  • 0270-6474

PubMed ID

  • 11007898
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Additional Document Info

start page

  • 7394

end page

  • 7403

volume

  • 20

issue

  • 19

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