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Immunolocalization and partial characterization of a nucleolar autoantigen (PM-Scl) associated with polymyositis/scleroderma overlap syndromes

Academic Article
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Overview

authors

  • Reimer, G.
  • Scheer, U.
  • Peters, J. M.
  • Tan, Eng

publication date

  • December 1986

journal

  • Journal of Immunology  Journal

abstract

  • Precipitating anti-PM-Scl antibodies are present in sera from patients with polymyositis, scleroderma, and polymyositis/scleroderma overlap syndromes. By indirect immunofluorescence microscopy, anti-PM-Scl antibodies stained the nucleolus in cells of different tissues and species, suggesting that the antigen is highly conserved. By electron microscopy, anti-PM-Scl antibodies reacted primarily with the granular component of the nucleolus. Drugs that inhibit rRNA synthesis had a marked effect on the expression of PM-Scl antigen. In actinomycin D-treated cells, immunofluorescence staining by anti-PM-Scl was significantly reduced with residual staining restricted to the granular regions of nucleoli. Treatment with 5,6-dichloro-beta-D-ribofuranosylbenzimidazole (DRB) also selectively reduced nucleolar staining. On a molecular level, anti-PM-Scl antibodies precipitated 11 polypeptides with molecular weights (Mr) ranging from 110,000 to 20,000. The Mr 80,000 and 20,000 polypeptides were phosphorylated. Evidence suggests that the PM-Scl antigen complex may be related to a preribosomal particle.

subject areas

  • Animals
  • Antigens, Nuclear
  • Autoantibodies
  • Autoantigens
  • Cell Cycle
  • Cell Nucleolus
  • Dactinomycin
  • Dichlororibofuranosylbenzimidazole
  • Fluorescent Antibody Technique
  • HeLa Cells
  • Humans
  • Myositis
  • Nucleoproteins
  • Scleroderma, Systemic
  • Vero Cells
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Identity

International Standard Serial Number (ISSN)

  • 0022-1767

PubMed ID

  • 3537125
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Additional Document Info

start page

  • 3802

end page

  • 3808

volume

  • 137

issue

  • 12

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