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Ganglioside gm2 as a human-tumor antigen (ofa-i-1)

Academic Article
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Overview

authors

  • Tai, T.
  • Paulson, James
  • Cahan, L. D.
  • Irie, R. F.

publication date

  • 1983

journal

  • Proceedings of the National Academy of Sciences of the United States of America-Biological Sciences  Journal

abstract

  • A monospecific antibody produced in vitro by a B-lymphoblastoid cell line transformed with Epstein-Barr virus has been shown to recognize a membrane antigen (OFA-I-1) on human tumors and fetal brain. This study identifies the chemical nature of OFA-I-1. The glycolipid fraction of antigen-rich spent medium of an OFA-I-1-positive melanoma cell line, M14, was extracted by chloroform/methanol/water, 4:8:3 (vol/vol), and was separated into fractions of neutral glycolipids and gangliosides by DEAE-Sephadex followed by base treatment and Bio-sil A column elution. OFA-I-1 antigens were found exclusively in the ganglioside fraction when assayed with monospecific anti-OFA-I-1 by an immune adherence inhibition test. The results obtained from thin-layer chromatography of the antigenic M14 ganglioside and sequential glycosidase digestion suggested that the antigen was a ganglioside GM2, GalNAc beta 1 leads to 4(NeuAc alpha 2 leads to 3)Gal beta 1 leads to 4Glc leads to Cer. These results were further supported by testing various authentic gangliosides and neutral glycolipids for OFA-I-1 antigenicity. Only GM2 showed positive reactivity.

subject areas

  • Adult
  • Antigens, Neoplasm
  • Brain
  • Carbohydrates
  • Cell Line
  • Epitopes
  • Female
  • Fetus
  • G(M2) Ganglioside
  • Gangliosides
  • Glycolipids
  • Humans
  • Liver
  • Melanoma
  • Pregnancy
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Identity

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.80.17.5392

PubMed ID

  • 6193515
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Additional Document Info

start page

  • 5392

end page

  • 5396

volume

  • 80

issue

  • 17

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