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The alpha-helical rod domain of human lamins A and C contains a chromatin binding site

Academic Article
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Overview

authors

  • Glass, C. A.
  • Glass, J. R.
  • Taniura, H.
  • Hasel, K. W.
  • Blevitt, J. M.
  • Gerace, Larry

publication date

  • November 1993

journal

  • EMBO Journal  Journal

abstract

  • We examined regions of human lamins A and C involved in binding to surfaces of mitotic chromosomes. An Escherichia coli expression system was used to produce full-length lamin A and lamin C, and truncated lamins retaining the central alpha-helical rod domain (residues 34-388) but lacking various amounts of the amino-terminal 'head' and carboxy-terminal 'tail' domains. We found that lamin A, lamin C and lamin fragments lacking the head domain and tail sequences distal to residue 431 efficiently assembled into paracrystals and strongly associated with mitotic chromosomes. Furthermore, the lamin rod domain also associated with chromosomes, although efficient chromosome coating required the pH 5-6 conditions needed to assemble the rod into higher order structures. Biochemical assays showed that chromosomes substantially reduced the critical concentration for assembly of lamin polypeptides into pelletable structures. Association of the lamin rod with chromosomes was abolished by pretrypsinization of chromosomes, and was not seen for vimentin (which possesses a similar rod domain). These data demonstrate that the alpha-helical rod of lamins A and C contains a specific chromosome binding site. Hence, the central rod domain of intermediate filament proteins can be involved in interactions with other cellular structures as well as in filament assembly.

subject areas

  • Animals
  • CHO Cells
  • Chromatin
  • Chromosomes
  • Cricetinae
  • DNA Mutational Analysis
  • Humans
  • Hydrogen-Ion Concentration
  • Intermediate Filaments
  • Lamin Type A
  • Lamins
  • Microscopy, Electron
  • Mitosis
  • Nuclear Proteins
  • Peptide Fragments
  • Protein Conformation
  • Recombinant Fusion Proteins
  • Structure-Activity Relationship
  • Trypsin
  • Vimentin
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Research

keywords

  • CHROMATIN BINDING
  • LAMINS
  • ROD DOMAIN
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Identity

PubMed Central ID

  • PMC413739

International Standard Serial Number (ISSN)

  • 0261-4189

PubMed ID

  • 8223451
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Additional Document Info

start page

  • 4413

end page

  • 4424

volume

  • 12

issue

  • 11

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