Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form

A role for nuclear factor kappa b/rel transcription factors in the regulation of the recombinase activator genes

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

related to degree

  • Duong, Bao Hoa, Ph.D. in Macromolecular and Cellular Structure and Chemistry, Scripps Research 2003 - 2009

authors

  • Verkoczy, L.
  • Ait-Azzouzene, D.
  • Skog, P.
  • Martensson, A.
  • Lang, J.
  • Duong, Bao Hoa
  • Nemazee, David

publication date

  • April 2005

journal

  • Immunity  Journal

abstract

  • In developing B cells, expression of surface immunoglobulin is an important signal to terminate recombinase activator gene (RAG) expression and V(D)J recombination. However, autoreactive antigen receptors instead promote continued gene rearrangement and receptor editing. The regulation by B cell receptor (BCR) signaling of RAG expression and editing is poorly understood. We report that in editing-competent cells BCR ligand-induced RAG mRNA expression is regulated at the level of RAG transcription, rather than mRNA stability. In immature B cells carrying innocuous receptors, RAG expression appears to be under rapidly reversible negative regulation. Studies involving transduction of a superrepressive (sr) I kappa B alpha protein indicate that NF-kappaB/Rel proteins promote RAG transcription. Interestingly, NF kappa B1-deficient cells overexpress RAG and undergo an exaggerated receptor editing response. Our data implicate NF kappa B transcription factors in the BCR-mediated regulation of RAG locus transcription. Rapidly activated NF kappa B pathways may facilitate prompt antigen receptor-regulated changes in RAG expression important for editing and haplotype exclusion.

subject areas

  • Animals
  • B-Lymphocytes
  • Base Sequence
  • Cell Line
  • DNA-Binding Proteins
  • Enhancer Elements, Genetic
  • Genes, RAG-1
  • Immunoglobulin kappa-Chains
  • Mice
  • Molecular Sequence Data
  • NF-kappa B
  • Protein Synthesis Inhibitors
  • RNA
  • Up-Regulation
scroll to property group menus

Identity

PubMed Central ID

  • PMC3792720

International Standard Serial Number (ISSN)

  • 1074-7613

Digital Object Identifier (DOI)

  • 10.1016/j.immuni.2005.03.006

PubMed ID

  • 15845455
scroll to property group menus

Additional Document Info

start page

  • 519

end page

  • 531

volume

  • 22

issue

  • 4

©2021 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support