Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form

Crystallization and preliminary structure determination of an intact human immunoglobulin, b12: An antibody that broadly neutralizes primary isolates of HIV-1

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

related to degree

  • Saphire, Erica Ollmann, Ph.D., Scripps Research 1994 - 2000

authors

  • Saphire, Erica Ollmann
  • Parren, P. W.
  • Barbas III, Carlos
  • Burton, Dennis
  • Wilson, Ian

publication date

  • January 2001

journal

  • Acta Crystallographica Section D-Biological Crystallography  Journal

abstract

  • An intact human immunoglobulin with a full-length hinge has been crystallized for the first time in a form in which all of the Ig domains are ordered. The IgG1 antibody b12 is one of only three known monoclonal antibodies described that potently neutralize a broad range of HIV-1 primary isolates. It binds to an epitope overlapping the conserved CD4 binding site on the viral surface antigen gp120. Hexagonal crystals corresponding to space group R32 were grown from 0.8 M ammonium sulfate, with unit-cell parameters a = b = 271.3, c = 175.2 A and one molecule per asymmetric unit. The crystals diffract to 2.8 A and a preliminary molecular-replacement solution indicates that all 12 Ig domains of the antibody can be resolved.

subject areas

  • Crystallization
  • HIV Infections
  • HIV-1
  • Humans
  • Immunoglobulins
  • Male
  • Neutralization Tests
  • Protein Conformation
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 0907-4449

Digital Object Identifier (DOI)

  • 10.1107/s0907444900017376

PubMed ID

  • 11134947
scroll to property group menus

Additional Document Info

start page

  • 168

end page

  • 171

volume

  • 57

issue

  • Pt 1

©2021 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support