Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form
As of April 1st VIVO Scientific Profiles will no longer updated for faculty, and the link to VIVO will be removed from the library website. Faculty profile pages will continue to be updated via Interfolio. VIVO will continue being used behind the scenes to update graduate student profiles. Please contact helplib@scripps.edu if you have questions.
How to download citations from VIVO | Alternative profile options

Vectors, promoters, and expression of genes in chick embryos

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Chen, H. Y.
  • Garber, E. A.
  • Mills, E.
  • Smith, J.
  • Kopchick, J. J.
  • Dilella, A. G.
  • Smith, Roy

publication date

  • 1990

journal

  • Journal of Reproduction and Fertility  Journal

abstract

  • Transgenic chickens were produced by injecting the Day-1 egg with 10(5) infectious particles of a replication-competent virus based on the Schmidt-Ruppin A strain of Rous sarcoma virus. The chickens were resistant to transforming subgroup A virus containing the src gene but not the corresponding subgroup B virus. Transgenic chickens producing bovine growth hormone (bGH) were generated using a modified virus containing the Bryan high titre polymerase gene. The virus was constructed with the bGH gene and the mouse metallothionein promoter in the reverse orientation relative to the viral structural genes. Two male chickens produced serum concentrations of approximately 100 ng bGH/ml; the birds were larger than controls and matured more rapidly. Transgenic mice required for the analysis of skeletal muscle-specific expression in vivo were produced using 5'-flanking regions of the chicken alpha-skeletal actin promoter linked to a luciferase reporter gene to determine the region essential for tissue-specific expression. The defined promoter sequences are to be used in experiments designed to direct expression of growth-promoting genes in skeletal muscle of chickens.

subject areas

  • Animals
  • Animals, Genetically Modified
  • Avian Sarcoma Viruses
  • Chick Embryo
  • Gene Expression
  • Genes, Viral
  • Genetic Vectors
  • Growth Hormone
  • Muscles
  • Promoter Regions, Genetic
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 0022-4251

PubMed ID

  • 2170640
scroll to property group menus

Additional Document Info

start page

  • 173

end page

  • 182

©2022 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support