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Interaction of urea with an unfolded protein - the DNA-binding domain of the 434-repressor

Academic Article
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Overview

authors

  • Dotsch, V.
  • Wider, G.
  • Siegal, G.
  • Wuthrich, Kurt

publication date

  • June 1995

journal

  • FEBS Letters  Journal

abstract

  • Experimental techniques are presented for the observation of the solvation of the unfolded form of a globular protein, the N-terminal 63-residue polypeptide from the 434 repressor, in 7 M aqueous urea solution by both water and urea. With the use of 15N-labelled urea it is demonstrated that the cross sections through two-dimensional nuclear Overhauser enhancement (NOE) spectra at the chemical shifts of H2O and urea both contain direct NOEs with the protein, under conditions where exchange peaks are observed only in the water cross section. A preliminary analysis of the data showed that the residence times of urea molecules in solvation sites near the methyl groups of Val, Leu and Ile are significantly longer than those of water molecules in the same sites.

subject areas

  • Amino Acids
  • Bacteriophage T7
  • Binding Sites
  • DNA-Binding Proteins
  • Magnetic Resonance Spectroscopy
  • Molecular Structure
  • Protein Binding
  • Protein Folding
  • Protons
  • Repressor Proteins
  • Solutions
  • Urea
  • Viral Proteins
  • Water
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Research

keywords

  • NMR
  • PROTEIN FOLDING
  • PROTEIN SOLVATION
  • UREA DENATURATION
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Identity

International Standard Serial Number (ISSN)

  • 0014-5793

Digital Object Identifier (DOI)

  • 10.1016/0014-5793(95)00459-m

PubMed ID

  • 7789518
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Additional Document Info

start page

  • 6

end page

  • 10

volume

  • 366

issue

  • 1

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