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An E. coli aminoacyl-tRNA synthetase can substitute for yeast mitochondrial enzyme function in vivo

Academic Article
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Overview

authors

  • Edwards, H.
  • Schimmel, Paul

publication date

  • November 1987

journal

  • Cell  Journal

abstract

  • We have investigated the function of an E. coli aminoacyl-tRNA synthetase in S. cerevisiae strains that are respiration-deficient because of a mutation or a gene disruption in the nuclear encoded gene for the mitochondrial tyrosyl-tRNA synthetase. Although the yeast mitochondrial and E. coli tyrosine tRNAs differ significantly in sequence, expression of the E. coli tyrosyl-tRNA synthetase from a gene fusion restores respiration. The fusion gene contains a presumptive sequence for mitochondrial import from the mitochondrial tyrosyl-tRNA synthetase gene fused to the E. coli coding region. The fusion protein is incorporated into mitochondria. This incorporation and the rescue of the respiratory defect require the presumptive sequence for mitochondrial import. These experiments suggest a more limited definition of the identity of a tyrosine tRNA.

subject areas

  • Amino Acyl-tRNA Synthetases
  • Bacterial Proteins
  • Escherichia coli
  • Fungal Proteins
  • Genetic Complementation Test
  • Mitochondria
  • RNA, Fungal
  • RNA, Transfer, Amino Acyl
  • Recombinant Fusion Proteins
  • Saccharomyces cerevisiae
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Identity

International Standard Serial Number (ISSN)

  • 0092-8674

Digital Object Identifier (DOI)

  • 10.1016/0092-8674(87)90133-4

PubMed ID

  • 3315228
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Additional Document Info

start page

  • 643

end page

  • 649

volume

  • 51

issue

  • 4

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